TNFSF14 Kit ELISA

N° du produit ABIN6720322

Détail du produit TNFSF14 Kit ELISA

Antigène: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD258, Delta transmembrane LIGHT, Herpes virus entry mediator ligand, Herpesvirus entry mediator A, Herpesvirus entry mediator ligand, HVEM L, HVEM-L, HVEML, Ligand for herpesvirus entry mediator, LTg, TNF14_HUMAN, Tnfsf14, Tumor necrosis factor (ligand) superfamily member 14, Tumor necrosis factor ligand superfamily member 14, Tumor necrosis factor receptor like 2, Tumor necrosis factor superfamily member LIGHT

Background: Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Gene Name: TNFSF14

Production: Natural and recombinant human TNFSF14. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D74-V240

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFSF14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or saliva to each empty well. It is recommended that each human TNFSF14 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Autre désignation: TNFSF14 - LIGHT (TNFSF14 Produits)

Synonymes: TNFSF14 Kit ELISA, CD258 Kit ELISA, HVEML Kit ELISA, LIGHT Kit ELISA, LTg Kit ELISA, TR2 Kit ELISA, HVEM-L Kit ELISA, Ly113 Kit ELISA, TNF superfamily member 14 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 14 Kit ELISA, TNFSF14 Kit ELISA, Tnfsf14 Kit ELISA

ID gène: 8740

NCBI Accession: NP_003798

UniProt: O43557

Pathways: Cancer Immune Checkpoints