COL18A1 Kit ELISA
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- Antigène Voir toutes COL18A1 Kits ELISA
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: Alpha 1 collagen type 18 (XVIII)(COL18A1), Alpha 1 type XVIII collagen, Antiangiogenic agent, COL18A1, Collagen alpha 1(XVIII) chain, Collagen type XVIII alpha 1, FLJ27325, FLJ34914, KNO, KNO1, MGC74745, Multi functional protein MFP, OTTMUSP00000023764, OTTMUSP00000023766, Procollagen type XVIII alpha 1
Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Gene Name: COL18A1
Production: Natural and recombinant human Endostatin. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: H1154-S1419
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or human milk to each empty well. It is recommended that each human Endostatin standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes COL18A1 Kits ELISA
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- Autre désignation
- Endostatin (COL18A1 Produits)
- Synonymes
- KNO Kit ELISA, KNO1 Kit ELISA, KS Kit ELISA, cb371 Kit ELISA, cb529 Kit ELISA, fc96d06 Kit ELISA, sb:cb529 Kit ELISA, wu:fb47h10 Kit ELISA, wu:fb77g12 Kit ELISA, wu:fc50f04 Kit ELISA, wu:fc96d06 Kit ELISA, wu:fk02h12 Kit ELISA, kno Kit ELISA, kno1 Kit ELISA, collagen type XVIII alpha 1 chain Kit ELISA, collagen type XVIII alpha 1 chain a Kit ELISA, collagen, type XVIII, alpha 1 Kit ELISA, collagen type XVIII alpha 1 Kit ELISA, COL18A1 Kit ELISA, col18a1a Kit ELISA, Col18a1 Kit ELISA, col18a1 Kit ELISA
- Classe de substances
- Chemical
- ID gène
- 80781
- NCBI Accession
- NP_085059
- UniProt
- P39060
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