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BAFF Kit ELISA
TNFSF13B
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730178
Détail du produit BAFF Kit ELISA
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Antigène
Voir toutes BAFF (TNFSF13B) Kits ELISA
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Reactivité
Toutes les réactivités sur BAFF Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse BAFF/BLyS/TNFSF13B ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse BAFF.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product TNFSF13B Kit ELISA
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes BAFF (TNFSF13B) Kits ELISA
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Autre désignation
BAFF (BLyS/TNFSF13B) (TNFSF13B Produits )
Synonymes
BAFF Kit ELISA, BLYS Kit ELISA, CD257 Kit ELISA, DTL Kit ELISA, TALL-1 Kit ELISA, TALL1 Kit ELISA, THANK Kit ELISA, TNFSF20 Kit ELISA, ZTNF4 Kit ELISA, BAFF-R Kit ELISA, BAFFR Kit ELISA, BROMIX Kit ELISA, CD268 Kit ELISA, CVID4 Kit ELISA, prolixin Kit ELISA, TNFSF13B Kit ELISA, RGD1561519 Kit ELISA, RGD1560810 Kit ELISA, BLyS Kit ELISA, D8Ertd387e Kit ELISA, zTNF4 Kit ELISA, 2010006P15Rik Kit ELISA, Baffr Kit ELISA, Bcmd Kit ELISA, Bcmd-1 Kit ELISA, Bcmd1 Kit ELISA, Lvis22 Kit ELISA, zgc:172115 Kit ELISA, TNF superfamily member 13b Kit ELISA, TNF receptor superfamily member 13C Kit ELISA, tumor necrosis factor (ligand) superfamily, member 13b Kit ELISA, tumor necrosis factor receptor superfamily, member 13c Kit ELISA, tumor necrosis factor superfamily member 13b Kit ELISA, TNFSF13B Kit ELISA, TNFRSF13C Kit ELISA, Tnfsf13b Kit ELISA, Tnfrsf13c Kit ELISA, tnfsf13b Kit ELISA
UniProt
Q9WU72
Pathways
Signalisation NF-kappaB , Production of Molecular Mediator of Immune Response
Vus récemment
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