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HVEM Kit ELISA
TNFRSF14
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730493
Détail du produit HVEM Kit ELISA
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Antigène
Voir toutes HVEM (TNFRSF14) Kits ELISA
HVEM (TNFRSF14)
(Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
Reactivité
Toutes les réactivités sur HVEM Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse HVEM/TNFRSF14 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse HVEM.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes HVEM (TNFRSF14) Kits ELISA
HVEM (TNFRSF14)
(Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
Autre désignation
HVEM (TNFRSF14 (TNFRSF14 Produits )
Synonymes
ATAR Kit ELISA, CD270 Kit ELISA, HVEA Kit ELISA, HVEM Kit ELISA, LIGHTR Kit ELISA, TR2 Kit ELISA, Atar Kit ELISA, HveA Kit ELISA, Hvem Kit ELISA, Tnfrs14 Kit ELISA, TNF receptor superfamily member 14 Kit ELISA, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Kit ELISA, Tnfrsf14 Kit ELISA, TNFRSF14 Kit ELISA
Classe de substances
Viral Protein
UniProt
Q80WM9
Pathways
Production of Molecular Mediator of Immune Response , Cancer Immune Checkpoints
Vus récemment
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