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UBE2W Kit ELISA

UBE2W Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730535
  • Antigène Tous les produits UBE2W
    UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human UBE2W ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human UBE2W.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Tous les produits UBE2W
    UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
    Autre désignation
    UBE2W (UBE2W Produits)
    Synonymes
    UBE2W Kit ELISA, im:6905459 Kit ELISA, si:ch211-193n21.4 Kit ELISA, ube2wa Kit ELISA, UBC-16 Kit ELISA, UBC16 Kit ELISA, 6130401J04Rik Kit ELISA, ubiquitin conjugating enzyme E2 W Kit ELISA, ubiquitin-conjugating enzyme E2W (putative) Kit ELISA, ubiquitin conjugating enzyme E2 W (putative) Kit ELISA, ubiquitin conjugating enzyme E2W (putative) Kit ELISA, ubiquitin-conjugating enzyme E2W Kit ELISA, UBE2W Kit ELISA, ube2w Kit ELISA, Ube2w Kit ELISA
    UniProt
    Q96B02
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