Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

IGFBP7 Kit ELISA

IGFBP7 Reactivité: Souris Colorimetric Sandwich ELISA 0.12 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN6730695
  • Antigène Voir toutes IGFBP7 Kits ELISA
    IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
    Reactivité
    • 5
    • 2
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.12 ng/mL - 25 ng/mL
    Seuil minimal de détection
    0.12 ng/mL
    Application
    ELISA
    Fonction
    Mouse IGFBP-rp1/IGFBP-7 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse IGFBP-rp1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product IGFBP7 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation des réactifs

    Recommended Dilution for serum and plasma samples20 fold

    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes IGFBP7 Kits ELISA
    IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
    Autre désignation
    IGFBP-rp1 (IGFBP-7 (IGFBP7 Produits)
    Synonymes
    zgc:85888 Kit ELISA, AGM Kit ELISA, FSTL2 Kit ELISA, IBP-7 Kit ELISA, IGFBP-7 Kit ELISA, IGFBP-7v Kit ELISA, IGFBPRP1 Kit ELISA, MAC25 Kit ELISA, PSF Kit ELISA, RAMSVPS Kit ELISA, TAF Kit ELISA, Fstl2 Kit ELISA, Mac25 Kit ELISA, insulin like growth factor binding protein 7 Kit ELISA, insulin-like growth factor binding protein 7 Kit ELISA, IGFBP7 Kit ELISA, igfbp7 Kit ELISA, Igfbp7 Kit ELISA
    UniProt
    Q61581
    Pathways
    Growth Factor Binding
Vous êtes ici:
Support technique