VISTA Kit ELISA

N° du produit ABIN6730768

Détail du produit VISTA Kit ELISA

Antigène: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3 ng/mL - 600 ng/mL

Seuil minimal de détection: 3 ng/mL

Application: ELISA

Fonction: Mouse VISTA/B7-H5/PD-1H ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse VISTA.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samples2 fold

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Autre désignation: VISTA (B7-H5/PD-1H) (VISTA Produits)

Synonymes: MGC112715 Kit ELISA, MGC151567 Kit ELISA, B7-H5 Kit ELISA, B7H5 Kit ELISA, GI24 Kit ELISA, PP2135 Kit ELISA, SISP1 Kit ELISA, Dies1 Kit ELISA, VISTA Kit ELISA, V-set immunoregulatory receptor Kit ELISA, vsir Kit ELISA, Vsir Kit ELISA, VSIR Kit ELISA

UniProt: Q9D659

Pathways: Cancer Immune Checkpoints