CHL1 Kit ELISA

N° du produit ABIN6730818

Détail du produit CHL1 Kit ELISA

Antigène: CHL1 (Cell Adhesion Molecule with Homology To L1CAM (Close Homolog of L1) (CHL1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse CHL-1/L1CAM-2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse CHL-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CHL1 (Cell Adhesion Molecule with Homology To L1CAM (Close Homolog of L1) (CHL1))

Autre désignation: CHL-1 (L1CAM-2 (CHL1 Produits)

Synonymes: CALL Kit ELISA, L1CAM2 Kit ELISA, CHL Kit ELISA, CHL1 Kit ELISA, Nrln1 Kit ELISA, VOPT Kit ELISA, A530023M13Rik Kit ELISA, AI465420 Kit ELISA, LICAM2 Kit ELISA, Call Kit ELISA, cell adhesion molecule L1 like Kit ELISA, chordin-like 1 Kit ELISA, cell adhesion molecule L1-like Kit ELISA, CHL1 Kit ELISA, Chrdl1 Kit ELISA, Chl1 Kit ELISA

UniProt: P70232