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HGF Kit IQ-ELISA

HGF Reactivité: Humain qPCR DNA-Binding ELISA 0.128 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN6730856
  • Antigène Voir toutes HGF IQ-ELISA Kits
    HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
    Reactivité
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    qPCR
    Type de méthode
    DNA-Binding ELISA
    Gamme de detection
    0.128 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    0.128 pg/mL
    Application
    ELISA
    Fonction
    Human Immunoquantitative (PCR-Based) HGF ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    0.128 pg/mL
    Attributs du produit
    The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human HGF IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human HGF in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human HGF coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
    Ingrédients
    • HGF Microplate (Item A): 96 well PCR plate coated with anti-Human HGF
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Human HGF
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for HGF (Item F): 2 vials of a 4x concentrated solution of anti-Human HGF affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Matériel non inclus
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
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  • Indications d'application
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Commentaires

    Features:
    - Excellent for follow up/confirmation of antibody array data
    - Pre-coated 96 well plate
    - Strip plate format minimizes waste
    - Complete kit includes all necessary reagents
    - 10x more sensitive than ELISA
    - Reproducible and reliable
    - Optimized for serum, plasma, urine and cell culture medium
    - 1/10th the sample volume of an ELISA

    Research Applications:
    - Detection of quantitative protein levels in biological fluids
    - Validation of antibody array results
    - Validation of biomarker discovery studies

    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Préparation des réactifs

    Recommended Dilution for serum and plasma samples: 2 - 10 fold

    Restrictions
    For Research Use only
  • Stockage commentaire
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.
    Date de péremption
    6 months
  • Antigène Voir toutes HGF IQ-ELISA Kits
    HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
    Autre désignation
    HGF (HGF Produits)
    Synonymes
    DFNB39 Kit IQ-ELISA, F-TCF Kit IQ-ELISA, HGFB Kit IQ-ELISA, HPTA Kit IQ-ELISA, SF Kit IQ-ELISA, HGF Kit IQ-ELISA, C230052L06Rik Kit IQ-ELISA, HGF/SF Kit IQ-ELISA, NK1 Kit IQ-ELISA, NK2 Kit IQ-ELISA, SF/HGF Kit IQ-ELISA, hepatocyte growth factor Kit IQ-ELISA, HGF Kit IQ-ELISA, Hgf Kit IQ-ELISA
    ID gène
    3082
    UniProt
    P14210
    Pathways
    Signalisation RTK, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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