TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag

N° du produit ABIN6809837

Détail du produit

Application: Immunoprecipitation (IP), Western Blotting (WB)

Fonction: TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag allows for the immunoprecipitation and detection of GFP-tagged recombinant proteins present in cell lysates

Marque: TrueBlot®

Specificité: This TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag allows for the detection of GFP-tagged recombinant protein present in cell lysates provided by the user. Mouse TrueBlot® ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.

Attributs du produit: TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag allows for the immunoprecipitation and detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of Mouse IgG TrueBlot® ULTRA HRP. The TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and western blot containing a GFP Epitope Tag using TrueBlot secondary antibody immunoblotting reagents in conjunction with Rockland's Anti-GFP (MOUSE) Monoclonal Antibody. Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance from interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Mouse IgG secondary antibody. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot® ULTRA.

Ingrédients: TrueBlot® IP and WB Kit for GFP Epitope Components:

Mouse IgG TrueBlot ULTRA: 50 μL ABIN1589976

TrueBlot Enhancer Solution: 25 mL

TrueBlot Blocker: 10 g

TrueBlot Assay Buffer: 30 mL 20X

TrueBlot Substrate A: 12.5 mL

TrueBlot Substrate B: 12.5 mL

Anti-Mouse Ig IP Beads: 2.5 mL Binds 0.4 mg Ig/mL beads

Anti-GFP (MOUSE) Monoclonal Antibody: 100 µg

Western Blot Incubation Tray.

Information d'application

Indications d'application: Western Blot : 1:1000
ImmunoPrecipitation: 1-10 μg / 10^7 cells/1 mL lysate

Commentaires:

Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). Mouse IgG TrueBlot® ULTRA is an HRP-conjugated monoclonal secondary antibody reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
Note that there are three key procedural considerations:

1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.
2. Immunoprecipitate should be completely reduced.
3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.

Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.

Restrictions: For Research Use only

Stockage

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Stock: 4 °C

Stockage commentaire: Store Kit at 2-8 °C, except Mouse TrueBlot® ULTRA and Anti-GFP (MOUSE) Monoclonal Antibody, which should be stored at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.