PLAP Kit ELISA

N° du produit ABIN6951070

Détail du produit PLAP Kit ELISA

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ALP ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Autre désignation: ALP (ALPP Produits)

Synonymes: ECK0378 Kit ELISA, JW0374 Kit ELISA, psiA Kit ELISA, ALP Kit ELISA, PALP Kit ELISA, PLAP Kit ELISA, PLAP-1 Kit ELISA, ILC Kit ELISA, CTACK Kit ELISA, ESkine Kit ELISA, Akp2 Kit ELISA, alp Kit ELISA, zgc:56672 Kit ELISA, DDBDRAFT_0205491 Kit ELISA, DDBDRAFT_0231570 Kit ELISA, DDB_0205491 Kit ELISA, DDB_0231570 Kit ELISA, ALPI Kit ELISA, Actn2lp Kit ELISA, Alp Kit ELISA, bacterial alkaline phosphatase Kit ELISA, alkaline phosphatase, placental Kit ELISA, alkaline phosphatase, placental type Kit ELISA, C-C motif chemokine ligand 27 Kit ELISA, alkaline phosphatase, liver/bone/kidney Kit ELISA, alkaline phosphatase Kit ELISA, alkaline phosphatase, intestinal Kit ELISA, PDZ and LIM domain 3 Kit ELISA, phoA Kit ELISA, ALPP Kit ELISA, LOC100436725 Kit ELISA, CCL27 Kit ELISA, alpl Kit ELISA, alp Kit ELISA, ALPI Kit ELISA, Pdlim3 Kit ELISA, Alpp Kit ELISA

ID gène: 250

UniProt: P05187