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PD-L1 Kit ELISA

PD-L1 Reactivité: Lapin Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951383
  • Antigène Voir toutes PD-L1 Kits ELISA
    PD-L1 (CD274 (PD-L1))
    Reactivité
    • 8
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Lapin
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rabbit PD-L1/CD274 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes PD-L1 Kits ELISA
    PD-L1 (CD274 (PD-L1))
    Autre désignation
    PD-L1 (CD274 (PD-L1 Produits)
    Synonymes
    B7-H Kit ELISA, B7H1 Kit ELISA, PD-L1 Kit ELISA, PDCD1L1 Kit ELISA, PDCD1LG1 Kit ELISA, PDL1 Kit ELISA, A530045L16Rik Kit ELISA, B7h1 Kit ELISA, Pdcd1l1 Kit ELISA, Pdcd1lg1 Kit ELISA, Pdl1 Kit ELISA, RGD1566211 Kit ELISA, CD274 molecule Kit ELISA, CD274 antigen Kit ELISA, programmed cell death 1 ligand 2 Kit ELISA, CD274 Kit ELISA, Cd274 Kit ELISA, PDCD1LG2 Kit ELISA
    ID gène
    100357106
    UniProt
    G1SUI3
    Pathways
    Cancer Immune Checkpoints
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