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IKKi/IKKe Kit ELISA

IKBKE Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951456
  • Antigène Voir toutes IKKi/IKKe (IKBKE) Kits ELISA
    IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human IKK-E/IKBKE ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes IKKi/IKKe (IKBKE) Kits ELISA
    IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
    Autre désignation
    IKK-E (IKBKE (IKBKE Produits)
    Synonymes
    zgc:100844 Kit ELISA, IKBKE Kit ELISA, MGC130907 Kit ELISA, IKK-E Kit ELISA, IKK-i Kit ELISA, IKKE Kit ELISA, IKKI Kit ELISA, AW558201 Kit ELISA, IKKepsilon Kit ELISA, Ikki Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit epsilon Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog Kit ELISA, inhibitor of kappaB kinase epsilon Kit ELISA, IKBKE Kit ELISA, ikbke Kit ELISA, ikbke.L Kit ELISA, Ikbke Kit ELISA
    ID gène
    9641
    UniProt
    Q14164
    Pathways
    Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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