TNFSF9 Kit ELISA
TNFSF9
Reactivité: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951520
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Antigène
Voir toutes TNFSF9 Kits ELISA
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TNFSF9
(Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
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Reactivité
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Rat
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Rat 4-1BB Ligand/TNFSF9 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes TNFSF9 Kits ELISA
-
TNFSF9
(Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
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Autre désignation
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4-1BB Ligand (TNFSF9 (TNFSF9 Produits)
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Synonymes
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TNFSF9 Kit ELISA, 4-1BB-L Kit ELISA, 4-1BBL Kit ELISA, AI848817 Kit ELISA, Cd137l Kit ELISA, Ly63l Kit ELISA, CD137L Kit ELISA, TNF superfamily member 9 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 9 Kit ELISA, Tnfsf9 Kit ELISA, LOC476729 Kit ELISA, TNFSF9 Kit ELISA
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ID gène
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353218
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UniProt
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Q80WE6
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Pathways
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Activated T Cell Proliferation, Cancer Immune Checkpoints
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