ITCH Kit ELISA

N° du produit ABIN6951536

Détail du produit ITCH Kit ELISA

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ITCH ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH (ITCH Produits)

Synonymes: AIF4 Kit ELISA, AIP4 Kit ELISA, NAPP1 Kit ELISA, dJ468O1.1 Kit ELISA, 6720481N21Rik Kit ELISA, 8030492O04Rik Kit ELISA, A130065M08 Kit ELISA, C230047C07Rik Kit ELISA, itchy E3 ubiquitin protein ligase Kit ELISA, itchy, E3 ubiquitin protein ligase Kit ELISA, ITCH Kit ELISA, Itch Kit ELISA

ID gène: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events