BTK Kit ELISA

N° du produit ABIN6951563

Détail du produit BTK Kit ELISA

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Épitope: pTyr551

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-BTK (Y551) Quantitative ELISA Kit. This assay quantitatively measures phosphorylated BTK (Tyr551) in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes human BTK phosphorylated at site Tyrosine-551.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Standard Protein

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or standard to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Standard Protein (Item S) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Autre désignation: BTK (BTK Produits)

Synonymes: BTK Kit ELISA, atk Kit ELISA, bpk Kit ELISA, xla Kit ELISA, imd1 Kit ELISA, agmx1 Kit ELISA, psctk1 Kit ELISA, AGMX1 Kit ELISA, AT Kit ELISA, ATK Kit ELISA, BPK Kit ELISA, IMD1 Kit ELISA, PSCTK1 Kit ELISA, XLA Kit ELISA, AI528679 Kit ELISA, xid Kit ELISA, Bruton tyrosine kinase Kit ELISA, Bruton agammaglobulinemia tyrosine kinase Kit ELISA, BTK Kit ELISA, btk Kit ELISA, Btk Kit ELISA

ID gène: 1133

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling