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LILRA5 Kit ELISA

LILRA5 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951737
  • Antigène Voir toutes LILRA5 Kits ELISA
    LILRA5 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 5 (LILRA5))
    Reactivité
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat LILRA5 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes LILRA5 Kits ELISA
    LILRA5 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 5 (LILRA5))
    Autre désignation
    LILRA5 (LILRA5 Produits)
    Synonymes
    CD85 Kit ELISA, CD85F Kit ELISA, ILT-11 Kit ELISA, ILT11 Kit ELISA, LILRB7 Kit ELISA, LIR-9 Kit ELISA, LIR9 Kit ELISA, E030017K20 Kit ELISA, EG232801 Kit ELISA, Gm4878 Kit ELISA, Lilrc1 Kit ELISA, leukocyte immunoglobulin like receptor A5 Kit ELISA, leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5 Kit ELISA, LILRA5 Kit ELISA, Lilra5 Kit ELISA
    ID gène
    691533
    UniProt
    D4A6Y0
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