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SARS-CoV-2 S1 Subunit (RBD) IgG Antibody Kit ELISA Kit ELISA

Reactivité: Humain, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Indirect ELISA Serum
N° du produit ABIN6952762
$464.20
Plus shipping costs $45.00
local_shipping Destination: Etats-Unis
Envoi sous 6 à 8 jours ouvrables
  • Antigène
    Reactivité
    Humain, SARS Coronavirus-2 (SARS-CoV-2)
    Méthode de détection
    Colorimetric
    Type de méthode
    Indirect ELISA
    Application
    ELISA
    Fonction
    COVID-19 S-Protein S1 Receptor Binding Domain Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum.
    Type d'échantillon
    Serum
    Analytical Method
    Quantitative
    Attributs du produit
    COVID-19 S-Protein (S1RBD) Human IgG ELISA Kit
    Ingrédients
    • SARS-CoV-2 S-protein S1RBD coated 96-well microplate
    • Wash Buffer
    • Positive Control
    • Negative Control
    • Biotinylated Anti-Human IgG
    • HRP-Streptavidin concentrate
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diluent
    • 5X Sample Diluent
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plaque
    Pre-coated
    Protocole
    This COVID19 human IgG antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 S protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for normalization purpose. The wells are washed and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 S protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    Procédure de l'essai
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
    3. Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
    4. Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
    5. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
    6. Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    12 months
  • Antigène
    Classe de substances
    Antibody
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