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Sonic Hedgehog Kit ELISA

SHH Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN6975950
  • Antigène Voir toutes Sonic Hedgehog (SHH) Kits ELISA
    Sonic Hedgehog (SHH)
    Reactivité
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    For the quantitative determination of human sonic hedgehog concentrations in serum, plasma, tissue homogenates, cell lysates.
    Type d'échantillon
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of human sonic hedgehog. No significant cross-reactivity or interference between human sonic hedgehog and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human sonic hedgehog and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    15.6 pg/mL
    Ingrédients
    • Assay plate
    • Standard
    • HRP-avidin (100 x concentrate)
    • Biotin-antibody (100 x concentrate)
    • Sample Diluent
    • HRP-avidin Diluent
    • Biotin-antibody Diluent
    • Wash Buffer (25 x concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip
    Top Product
    Discover our top product SHH Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare reagents, samples and standards as instructed.
    2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
    3. Remove the liquid of each well, don't wash.
    4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
    5. Aspirate and wash 3 times.
    6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
    7. Aspirate and wash 5 times.
    8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
    9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.
    Préparation des réactifs
    1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
    2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
    3. Wash Buffer(1x)- If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
    4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 4000 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undi luted Standard serves as the high standard (4000 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25 °C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
    • Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.
    Préparation de l'échantillon
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Précision du teste
    Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
    Date de péremption
    6 months
  • Filges, Röthlisberger, Blattner, Boesch, Demougin, Wenzel, Huber, Heinimann, Weber, Miny: "Deletion in Xp22.11: PTCHD1 is a candidate gene for X-linked intellectual disability with or without autism." dans: Clinical genetics, Vol. 79, Issue 1, pp. 79-85, (2011) (PubMed).

  • Antigène Voir toutes Sonic Hedgehog (SHH) Kits ELISA
    Sonic Hedgehog (SHH)
    Autre désignation
    sonic hedgehog homolog (Drosophila) (SHH Produits)
    Synonymes
    HHG1 Kit ELISA, HLP3 Kit ELISA, HPE3 Kit ELISA, MCOPCB5 Kit ELISA, SMMCI Kit ELISA, TPT Kit ELISA, TPTPS Kit ELISA, 9530036O11Rik Kit ELISA, Dsh Kit ELISA, Hhg1 Kit ELISA, Hx Kit ELISA, Hxl3 Kit ELISA, M100081 Kit ELISA, fc83d08 Kit ELISA, shh Kit ELISA, syu Kit ELISA, vhh-1 Kit ELISA, vhh1 Kit ELISA, wu:fc83d08 Kit ELISA, Xhh Kit ELISA, hedgehog Kit ELISA, xshh Kit ELISA, SHH Kit ELISA, twh Kit ELISA, twhh Kit ELISA, sonic hedgehog Kit ELISA, sonic hedgehog a Kit ELISA, sonic hedgehog L homeolog Kit ELISA, sonic hedgehog protein A Kit ELISA, sonic hedgehog b Kit ELISA, SHH Kit ELISA, Shh Kit ELISA, shha Kit ELISA, shh.L Kit ELISA, shh Kit ELISA, shhb Kit ELISA
    Sujet

    Abbreviation: SHH

    Alias: HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, sonic hedgehog

    UniProt
    Q15465
    Pathways
    Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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