FAS Kit ELISA

N° du produit ABIN921055

Détail du produit FAS Kit ELISA

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Épitope: AA 22-169

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Soluble FAS

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21
Immunogen sequence: Q22-R169

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <3pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: Q22-R169

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse FAS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FAS has been precoated onto 96-well plates. Standards(sf21, Q22-R169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FAS amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FAS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FAS standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 107, Standard deviation: 9.52, CV(%): 8.9
• Sample 2: n=16, Mean(pg/ml): 728, Standard deviation: 30.58, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 1407, Standard deviation: 54.87, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 116, Standard deviation: 10.56, CV(%): 9.1
• Sample 2: n=24, Mean(pg/ml): 739, Standard deviation: 40.65, CV(%): 5.5
• Sample 3: n=24, Mean(pg/ml): 1412, Standard deviation: 80.48, CV(%): 5.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour FAS Kit ELISA (ABIN921055)

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." dans: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." dans: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, (2014) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." dans: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." dans: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." dans: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

Détail du antigène

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: FAS (FAS Produits)

Sujet:

Background: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48- kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.

Synonyms: Tumor necrosis factor receptor superfamily member 6 ,Fas ,

Full Gene Name: Fas cell surface death receptor

ID gène: 14102

UniProt: Q8C350

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity