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Transferrin Kit ELISA

TF Reactivité: Souris Colorimetric Sandwich ELISA
N° du produit ABIN956035
  • Antigène Voir toutes Transferrin (TF) Kits ELISA
    Transferrin (TF)
    Reactivité
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    The Mouse Transferrin ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative determination of Transferrin in mouse biological samples.
    Analytical Method
    Quantitative
    Attributs du produit
    This ELISA can be used to measure transferrin in serum, tissue extracts and other biological fluids.In this assay the transferrin present in samples reacts with the anti-transferrin antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-transferrin antibodies conjugated with horseradish peroxidase (HRP), are added. This HRP-conjugated antibody forms a complex with the previously bound transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme is proportional to the concentration of transferrin in the sample tested, thus, the absorbance, at 450 nm, is a measure of the concentration of transferrin in the test sample. The quantity of transferrin in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution.
    Ingrédients
    1. Diluent Concentrate: One bottle containing 50 mL of a 5X concentrated diluent running buffer.
    2. Wash Solution Concentrate: One bottle containing 50 mL of a 20X concentrated wash solution.
    3. Enzyme-Antibody Conjugate Concentrate: One vial containing 150 µL of a 100X concentrated affinity-purified anti-mouse transferrin antibody conjugated with HRP in a stabilizing buffer.
    4. TMB Substrate Solution: One vial containing 12 mL of TMB and hydrogen peroxide in citric acid buffer at pH 3.3.
    5. Stop Solution: One vial containing 12 mL of 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
    6. Microtiter Plate: Twelve removable eight-well strips in well holder frame. Wells are coated with affinity-purified anti-mouse transferrin.
    7. Mouse Transferrin Calibrator: One vial containing a lyophilized Mouse Transferrin Calibrator.
    8. Positive Control: One vial containing 50 µL of serum with 0.1% sodium azide. See the Control Certificate for the concentration.
    Matériel non inclus
    Test tubes
    Precision pipettes (2 µL to 200 µL) for making and dispensing dilutions
    Microplate washer/aspirator
    Distilled or de-ionized H2O Microplate reader
    Assorted glassware for the preparation of reagents and buffer solutions
    Timer
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  • Plaque
    Pre-coated
    Préparation des réactifs
    1. Diluent Concentrate: The Diluent solution supplied is a 5X concentrate and must be diluted 1:5 with distilled or de-ionized water.
      2. Wash Solution Concentrate: The Wash Solution supplied is a 20X concentrate and must be diluted 1:20 with distilled or de-ionized water. Crystal formation in the concentrate is not uncommon when storage temperatures are low. Warming of the concentrate to 30-35°C before dilution can dissolve crystals.
      3. Enzyme-Antibody Conjugate Concentrate: Calculate the required amount of working conjugate solution for each microtiter plate test strip by adding 10 µL Enzyme-Antibody Conjugate to 990 µL of 1X Diluent for each test strip to be used for testing. Mix uniformly, but gently. Avoid foaming.
      4. TMB Substrate Solution: Ready to use as supplied.
      5. Stop Solution: Ready to use as supplied.
      6. Microtiter Plate: Ready to use as supplied. Unseal Microtiter Pouch and remove plate from pouch. Remove all strips and wells that will not be used in the assay and place back in pouch and re-seal along with desiccant.
      7. Mouse Transferrin Calibrator: Add 1.0 mL of distilled or de-ionized water to the lyophilized Mouse Transferrin Calibrator and mix gently until dissolved. The calibrator is now at a concentration of 8.86 µg/mL (the reconstituted calibrator should be aliquoted and frozen if future use is intended). Prepare the Mouse Transferrin Calibrators immediately prior to use according to the table below. Mix well between each step. Avoid foaming.
      8. Positive Control: The concentration and recommended dilution are provided on the Control Certificate. Before use, briefly centrifuge the Positive Control to allow all of the liquid to collect in the bottom of the vial.
    Calcul des résultats
    1. Subtract the average background value from the test values for each sample.
      2. Using the results observed for the calibrators construct a calibration curve. The appropriate curve fit is that of a four- parameter logistics curve. A second order polynomial (quadratic) or other curve fits may also be used.
      3. Interpolate test sample values from the calibration curve. Correct for sample dilution factor to arrive at transferrin concentration in original sample.

      1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the information contained in the package insert instructions and with adherence to good laboratory practice.
      2. Factors that might affect the performance of the assay include proper instrument function, cleanliness of glassware, quality of distilled or de-ionized water, washing thoroughly and accuracy of reagent and sample pipettings, washing technique, incubation time or temperature.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    1. Complete Kit: The expiration date for the kit is stated on the outer label. The recommended storage temperature is 4°C. Note: See long-term storage recommendations below for the Mouse Transferrin Calibrator and Positive Control.
    2. Diluent: The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4°C.
    3. Wash Solution: The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (RT, 16-25°C) or at 4°C.
    4. Enzyme-Antibody Conjugate: Undiluted anti-transferrin-HRP conjugate should be stored at 4°C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark.
    5. TMB Substrate Solution: The TMB Substrate Solution should be stored at 4°C and is stable until the expiration date.
    6. Stop Solution: The Stop Solution should be stored at 4°C and is stable until the expiration date.
    7. Microtiter Plate: Anti-mouse transferrin coated wells are stable until the expiration date and should be stored at 4°C in the sealed foil pouch with a desiccant pack.
    8. Mouse Transferrin Calibrator: The lyophilized Mouse Transferrin Calibrator should be stored at 4°C or frozen until reconstituted. The reconstituted calibrator should be aliquoted and stored frozen. Avoid multiple freeze/thaw cycles. The working calibrator solutions should be prepared immediately prior to use and are stable for up to 8 hours.
    9. Positive Control: For storage longer than 7 days keep frozen until the expiration date. Storage less than 7 days can be at 4°C. Avoid multiple freeze/thaw cycles.
    Date de péremption
    The expiry date is stated on the label.
  • Antigène Voir toutes Transferrin (TF) Kits ELISA
    Transferrin (TF)
    Autre désignation
    Transferrin (TF Produits)
    Synonymes
    ltf Kit ELISA, pro1557 Kit ELISA, pro2086 Kit ELISA, mgc107777 Kit ELISA, LOC692564 Kit ELISA, TF Kit ELISA, LOC100144362 Kit ELISA, tf Kit ELISA, LTF Kit ELISA, TFEW Kit ELISA, conalbumin Kit ELISA, tf-b Kit ELISA, MGC64306 Kit ELISA, PRO1557 Kit ELISA, PRO2086 Kit ELISA, TFQTL1 Kit ELISA, AI266983 Kit ELISA, Cd176 Kit ELISA, HP Kit ELISA, Tf Kit ELISA, Tfn Kit ELISA, hpx Kit ELISA, Trf Kit ELISA, cb285 Kit ELISA, gavi Kit ELISA, id:ibd3238 Kit ELISA, id:ibd3525 Kit ELISA, sb:cb285 Kit ELISA, wu:fb57g06 Kit ELISA, wu:fb62h02 Kit ELISA, wu:fb63h10 Kit ELISA, wu:fb64h10 Kit ELISA, zgc:112154 Kit ELISA, 143958_at Kit ELISA, CG6186 Kit ELISA, Dmel\\CG6186 Kit ELISA, TSF1 Kit ELISA, anon-EST:Posey265 Kit ELISA, tsf1 Kit ELISA, Pro-TRH Kit ELISA, IL-5 Kit ELISA, STF I Kit ELISA, TRF1 Kit ELISA, sTF1 Kit ELISA, sTf Kit ELISA, tf1 Kit ELISA, transferrin Kit ELISA, serotransferrin Kit ELISA, transferrin (ovotransferrin) Kit ELISA, transferrin L homeolog Kit ELISA, melanotransferrin Kit ELISA, transferrin-a Kit ELISA, Transferrin 1 Kit ELISA, thyrotropin releasing hormone Kit ELISA, interleukin 5 Kit ELISA, TF Kit ELISA, LOC477072 Kit ELISA, tf Kit ELISA, Tf Kit ELISA, LOC100144362 Kit ELISA, tf.L Kit ELISA, LOC5575625 Kit ELISA, Trf Kit ELISA, tfa Kit ELISA, Tsf1 Kit ELISA, TRH Kit ELISA, IL5 Kit ELISA, LOC101085148 Kit ELISA, LOC100726872 Kit ELISA, trf Kit ELISA
    Sujet
    Transferrin is a metal-combining protein that reversibly binds to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participates in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, transferrin is synthesized in the liver, but unlike haptoglobin, transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system.
    Pathways
    Transition Metal Ion Homeostasis
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