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EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

EDN1 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
N° du produit ABIN1339705
  • Antigène Tous les produits Endothelin 1 (EDN1)
    Endothelin 1 (EDN1)
    Espèce de la protéine
    Humain
    Espèce du lysat
    Human Cells
    Application
    Western Blotting (WB), Immunoprecipitation (IP)
    Fonction
    Transient Overexpression Lysate
    Specificité
    Non-denatured
    Attributs du produit
    EDN1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
    Used Plasmid: pCMV-EDN1 full length
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • Indications d'application
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Commentaires

    Product Quality tested by: Transient overexpression cell lysate was tested with Anti-EDN1 antibody by Western Blots.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2 mg/mL
    Buffer
    Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    Conseil sur la manipulation
    Aliquot to avoid repeated freezing and thawing.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • Antigène
    Endothelin 1 (EDN1)
    Autre désignation
    EDN1 (EDN1 Produits)
    Synonymes
    et1 Lysate, et-1 Lysate, Edn1 Lysate, EDN Lysate, ET-1 Lysate, ET1 Lysate, PPET1 Lysate, HDLCQ7 Lysate, preproET Lysate, Et1 Lysate, fb14d01 Lysate, wu:fb14d01 Lysate, PPET-1 Lysate, EDN1 Lysate, edn1 Lysate, edn1-a Lysate, edn1-b Lysate, endothelin 1 Lysate, endothelin 1 S homeolog Lysate, endothelin 1 L homeolog Lysate, edn1 Lysate, EDN1 Lysate, Edn1 Lysate, edn1.S Lysate, edn1.L Lysate
    Sujet
    Full Gene Name: endothelin 1
    Synonyms: ET1,HDLCQ7
    ID gène
    1906
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