ASAH1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
ASAH1
WB, IP
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivité: Humain
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- Antigène Tous les produits ASAH1
- ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))
- Espèce de la protéine
- Humain
- Espèce du lysat
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Fonction
- Transient Overexpression Lysate
- Specificité
- Non-denatured
- Attributs du produit
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ASAH1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-ASAH1 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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ASAH1 293T Cell Transient Overexpression Lysate(Denatured)
ASAH1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
-
- Indications d'application
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Commentaires
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-ASAH1 antibody by Western Blots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Conseil sur la manipulation
- Aliquot to avoid repeated freezing and thawing.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Antigène
- ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))
- Autre désignation
- ASAH1 (ASAH1 Produits)
- Synonymes
- AC Lysate, ACDase Lysate, ASAH Lysate, PHP Lysate, PHP32 Lysate, SMAPME Lysate, 2310081N20Rik Lysate, Asah Lysate, MGC82286 Lysate, asah1 Lysate, zgc:101637 Lysate, ASAH1 Lysate, zgc:66026 Lysate, N-acylsphingosine amidohydrolase 1 Lysate, N-acylsphingosine amidohydrolase (acid ceramidase) 1 Lysate, N-acylsphingosine amidohydrolase (acid ceramidase) 1 L homeolog Lysate, N-acylsphingosine amidohydrolase (acid ceramidase) 1a Lysate, Acid ceramidase Lysate, N-acylsphingosine amidohydrolase (acid ceramidase) 1b Lysate, ASAH1 Lysate, Asah1 Lysate, asah1.L Lysate, asah1a Lysate, asah-1 Lysate, asah1b Lysate
- Sujet
-
Full Gene Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
Synonyms: AC,ASAH,FLJ21558,FLJ22079,PHP,PHP32 - ID gène
- 427
- NCBI Accession
- NP_808592, NM_177924
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