anti-Humain Dynactin 1 anticorps pour Flow Cytometry

Recommended Dynactin 1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Dynactin 1 (DCTN1) Anticorps
  • DAP-150
  • DP-150
  • P135
  • Chip150
  • DCTN1
  • DKFZp469C1813
  • AL022633
  • Glued
  • p150
  • p150
  • CG9206
  • DYNA_DROME
  • Dmel\\CG9206
  • FCP-D
  • Glu
  • P150
  • P150[Glued]
  • p150/Glued
  • p150/glued
  • p150[Glued]
  • p150[glued]
  • t1
  • Dynactin-1
  • glued
  • dynactin subunit 1
  • dynactin 1
  • Dynactin 1, p150 subunit
  • dynactin subunit 1 L homeolog
  • DCTN1
  • Dctn1
  • DCTN1-p150
  • dctn1.L
Épitope
C-Term
21
8
5
4
4
2
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
99
56
45
6
5
4
3
2
2
2
2
1
1
1
Hôte
Chèvre
58
23
17
4
Clonalité
Polyclonal
Conjugué
Cet anticorp Dynactin 1 est non-conjugé
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
87
42
41
16
13
11
8
7
3
1
1
1
Options
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N° du produit ABIN184834
$ 429.84
Plus shipping costs $45.00

General

Antigène Dynactin 1 (DCTN1) Anticorps
Épitope C-Term
(21), (8), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris
(99), (56), (45), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Hôte Chèvre
(58), (23), (17), (4)
Clonalité
Polyclonal
Conjugué Cet anticorp Dynactin 1 est non-conjugé
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(87), (42), (41), (16), (13), (11), (8), (7), (3), (1), (1), (1)
Pubmed 2 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-Dynactin 1 anticorps

Détail du antigène Dynactin 1 Information d'application Stockage References for anti-Dynactin 1 antibody (ABIN184834) Images
Fonction Dynactin
Blocking Peptide Blocking peptide for this product available: ABIN369622
Séquence QEQLHQLHSR LIS
Specificité This antibody is expected to recognise all reported isoforms.
Homologie Human, Mouse
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Classe de qualité Verified
Immunogène Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.
Plasmids, Primers & others

Détail du antigène Dynactin 1

Détail du produit anti-Dynactin 1 anticorps Information d'application Stockage References for anti-Dynactin 1 antibody (ABIN184834) Images Haut de la page
Antigène
Autre désignation DCTN1 (DCTN1 Antibody Extrait)
Sujet DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog
ID gène 1639
NCBI Accession NP_004073, NP_075408, NP_001128512, NP_001128513, NP_001177765, NP_001177766
Pathways M Phase, ER-Nucleus Signaling

Information d'application

Détail du produit anti-Dynactin 1 anticorps Détail du antigène Dynactin 1 Stockage References for anti-Dynactin 1 antibody (ABIN184834) Images Haut de la page
Indications d'application

DS WB Results: Approx 150 kDa band observed in Human Cerebellum lysates (calculated MW of 142 kDa according to NP_004073.2). Additional bands between approx. 40-70 kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.

DS IHC Results: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4 μg/mL. A customer found positive results in IHC on Human HeLa cells.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml. Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Stockage

Détail du produit anti-Dynactin 1 anticorps Détail du antigène Dynactin 1 Information d'application References for anti-Dynactin 1 antibody (ABIN184834) Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20 °C.

References for anti-Dynactin 1 antibody (ABIN184834)

Détail du produit anti-Dynactin 1 anticorps Détail du antigène Dynactin 1 Information d'application Stockage Images Haut de la page
Produit citée dans:

Bupha-Intr, Haizlip, Janssen: "Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." dans: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, 2009

Background publications

Holzbaur, Tokito: "Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization." dans: Genomics, Vol. 31, Issue 3, pp. 398-9, 1997

Images

Détail du produit anti-Dynactin 1 anticorps Détail du antigène Dynactin 1 Information d'application Stockage References for anti-Dynactin 1 antibody (ABIN184834) Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Dynactin 1 (DCTN1) (C-Term) antibody (ABIN184834) ABIN184834 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein ...
Immunohistochemistry (IHC) image for anti-Dynactin 1 (DCTN1) (C-Term) antibody (ABIN184834) ABIN184834 (2.5µg/ml) staining of A) paraffin embedded Human Cerebellum and B) paraff...
Immunohistochemistry (IHC) image for anti-Dynactin 1 (DCTN1) (C-Term) antibody (ABIN184834) ABIN184834 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antige...