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Envoyez-nous votre proposition de validation. J'aimerais valider ce produitRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Fournisseur | Clonality | References | Details |
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11.745464 | ABIN152736 | FACS ICC IF IHC IHC (fro) IHC (p) IP WB | Mouse | IgG2a | Connectez-vous pour afficher | 2A7-A1 | 11 |
General |
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Antigène | ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Anticorps |
Reactivité | Amphibien, Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Porc, Primate, Lapin, Rat (Rattus), Mouton, Virus Alternatives |
Hôte | Souris Alternatives |
Clonalité (Clone) | |
Conjugué | Cet anticorp ATP2A2 est non-conjugé Alternatives |
Application |
Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
Alternatives
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16 références disponible |
Fournisseur | Connectez-vous pour afficher |
Détail du produit anti-ATP2A2 anticorpsDétail du antigène ATP2A2 Information d'application Stockage References for anti-ATP2A2 antibody (ABIN152688) Images |
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Specificité | Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This does not discriminate between the two isoforms. |
Purification | Immunogen affinity purified |
Immunogène | Purified canine cardiac sarcoplasmic reticulum. |
Clone | IID8 |
Isotype | IgG1 |
Détail du antigène ATP2A2Détail du produit anti-ATP2A2 anticorps Information d'application Stockage References for anti-ATP2A2 antibody (ABIN152688) Images Haut de la page |
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Antigène | |
Autre désignation | ATP2A2 / SERCA2 (ATP2A2 Antibody Extrait) |
Classe de substances | Virus |
Sujet | Gene Symbol: ATP2A2 |
ID gène | 488 |
UniProt | O46674 |
Pathways | Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process |
Information d'applicationDétail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Stockage References for anti-ATP2A2 antibody (ABIN152688) Images Haut de la page |
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Indications d'application | Western Blot 1:500 - 1:5000, Flow Cytometry 1 μg/ 10^6 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:200, Immunohistochemistry-Paraffin 1 μg/mL, Immunohistochemistry-Frozen 1:500, Blocking/Neutralizing 50 - 200 Molar excessWB: Detects an approx. 110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Not recommended for WB detection of rat SERCA2. IF: Staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. May be useful in Flow. |
Commentaires |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
StockageDétail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Information d'application References for anti-ATP2A2 antibody (ABIN152688) Images Haut de la page |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer |
PBS Buffer contains: 0.05 % Sodium Azide |
Agent conservateur | Sodium azide |
Précaution d'utilisation | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Conseil sur la manipulation | Avoid freeze-thaw cycles |
Stock | -20 °C |
Stockage commentaire | Store at -20°C. Avoid freeze-thaw cycles. |
References for anti-ATP2A2 antibody (ABIN152688)Détail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Information d'application Stockage Images Haut de la page |
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Produit citée dans: |
Leatherbury, Yu, Chatterjee, Walker, Yu, Tian, Lo: "A novel mouse model of X-linked cardiac hypertrophy." dans: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 6, pp. H2701-11, 2008 Chauvet, Tian, Husson, Grimm, Wang, Hiesberger, Hieseberger, Igarashi, Bennett, Ibraghimov-Beskrovnaya, Somlo, Caplan: "Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus." dans: The Journal of clinical investigation, Vol. 114, Issue 10, pp. 1433-43, 2004 Kjellgren, Ryan, Ohlendieck, Thornell, Pedrosa-Domellöf: "Sarco(endo)plasmic reticulum Ca2+ ATPases (SERCA1 and -2) in human extraocular muscles." dans: Investigative ophthalmology & visual science, Vol. 44, Issue 12, pp. 5057-62, 2003 Adachi, Matsui, Xu, Kirber, Lazar, Sharov, Schöneich, Cohen: "Antioxidant improves smooth muscle sarco/endoplasmic reticulum Ca(2+)-ATPase function and lowers tyrosine nitration in hypercholesterolemia and improves nitric oxide-induced relaxation." dans: Circulation research, Vol. 90, Issue 10, pp. 1114-21, 2002 Molnar, Seidler, Jona, Martonosi: "The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules." dans: Biochimica et biophysica acta, Vol. 1023, Issue 2, pp. 147-67, 1990 |
Background publications |
Poch, Leach, Snape, Cacic, MacLennan, Lytton: "Functional characterization of alternatively spliced human SERCA3 transcripts." dans: The American journal of physiology, Vol. 275, Issue 6 Pt 1, pp. C1449-58, 1999 Hu, Yin, Zhang, Wright, Nixon, Wechsler, Spratt, Briggs: "Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching." dans: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11619-22, 1995 Sharp, McPherson, Dawson, Aoki, Campbell, Snyder: "Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 7, pp. 3051-63, 1993 Molnar, Varga, Jona, Seidler, Martonosi: "Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase." dans: Biochimica et biophysica acta, Vol. 1103, Issue 2, pp. 281-95, 1992 Ohlendieck, Briggs, Lee, Wechsler, Campbell: "Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle." dans: European journal of biochemistry / FEBS, Vol. 202, Issue 3, pp. 739-47, 1992 Plessers, Eggermont, Wuytack, Casteels: "A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 11, Issue 3, pp. 650-6, 1991 Spencer, Yu, Khan, Grover: "Expression of isoforms of internal Ca2+ pump in cardiac, smooth muscle and non-muscle tissues." dans: Biochimica et biophysica acta, Vol. 1063, Issue 1, pp. 15-20, 1991 Ohlendieck, Ervasti, Snook, Campbell: "Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma." dans: The Journal of cell biology, Vol. 112, Issue 1, pp. 135-48, 1991 Movsesian, Leveille, Krall, Colyer, Wang, Campbell: "Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles." dans: Journal of molecular and cellular cardiology, Vol. 22, Issue 12, pp. 1477-85, 1991 Briggs, Lee, Feher, Wechsler, Ohlendieck, Campbell: "Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle." dans: FEBS letters, Vol. 259, Issue 2, pp. 269-72, 1990 Jorgensen, Arnold, Pepper, Kahl, Mandel, Campbell: "A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study." dans: Cell motility and the cytoskeleton, Vol. 9, Issue 2, pp. 164-74, 1988 |
ImagesDétail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Information d'application Stockage References for anti-ATP2A2 antibody (ABIN152688) Haut de la page |
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