anti-Boeuf (Vache) ATP2A2 anticorps pour Western Blotting

Recommended ATP2A2 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Anticorps
  • atp2b
  • ca-p60a
  • dar
  • serca2
  • ATP2A2
  • ATP2B
  • DAR
  • DD
  • SERCA2
  • SERCA2A
  • ATP2
  • Serca2
  • SercaII
  • 9530097L16Rik
  • D5Wsu150e
  • SERCA2B
  • mKIAA4195
  • atp2a2
  • zgc:55380
  • ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 S homeolog
  • ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
  • ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
  • ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a
  • atp2a1.S
  • ATP2A2
  • Atp2a2
  • atp2a2a
Reactivité
Amphibien, Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Porc, Primate, Lapin, Rat (Rattus), Mouton, Virus
68
41
39
11
7
6
6
5
4
4
3
2
2
2
2
2
1
1
1
1
Hôte
Souris
65
6
2
Clonalité
Monoclonal
Conjugué
Cet anticorp ATP2A2 est non-conjugé
3
3
3
2
2
2
Application
Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
70
34
22
11
7
6
3
2
1
1
1
Fournisseur
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N° du produit ABIN152688
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
3.0807028 ABIN2782136 IHC WB Rabbit C-Term Connectez-vous pour afficher Polyclonal 1
1 ABIN2617318 IHC IHC (p) WB Rabbit IgG AA 794-843 Connectez-vous pour afficher Polyclonal 0
1 ABIN2738411 IHC IHC (p) WB Rabbit IgG AA 1-32 Connectez-vous pour afficher Polyclonal 0
1 ABIN1976863 IP WB Rabbit IgG AA 475-525 Connectez-vous pour afficher Polyclonal 0

General

Antigène ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Anticorps
Reactivité Amphibien, Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Porc, Primate, Lapin, Rat (Rattus), Mouton, Virus
(68), (41), (39), (11), (7), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Hôte Souris
(65), (6), (2)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp ATP2A2 est non-conjugé
(3), (3), (3), (2), (2), (2)
Application Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
(70), (34), (22), (11), (7), (6), (3), (2), (1), (1), (1)
Pubmed 16 références disponible
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Détail du produit anti-ATP2A2 anticorps

Détail du antigène ATP2A2 Information d'application Stockage References for anti-ATP2A2 antibody (ABIN152688) Images
Specificité Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This does not discriminate between the two isoforms.
Purification Immunogen affinity purified
Immunogène Purified canine cardiac sarcoplasmic reticulum.
Clone IID8
Isotype IgG1
Plasmids, Primers & others

Détail du antigène ATP2A2

Détail du produit anti-ATP2A2 anticorps Information d'application Stockage References for anti-ATP2A2 antibody (ABIN152688) Images Haut de la page
Antigène
Autre désignation ATP2A2 / SERCA2 (ATP2A2 Antibody Extrait)
Classe de substances Virus
Sujet Gene Symbol: ATP2A2
ID gène 488
UniProt O46674
Pathways Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process

Information d'application

Détail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Stockage References for anti-ATP2A2 antibody (ABIN152688) Images Haut de la page
Indications d'application Western Blot 1:500 - 1:5000, Flow Cytometry 1 μg/ 10^6 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:200, Immunohistochemistry-Paraffin 1 μg/mL, Immunohistochemistry-Frozen 1:500, Blocking/Neutralizing 50 - 200 Molar excessWB: Detects an approx. 110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Not recommended for WB detection of rat SERCA2. IF: Staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. May be useful in Flow.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Stockage

Détail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Information d'application References for anti-ATP2A2 antibody (ABIN152688) Images Haut de la page
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -20 °C
Stockage commentaire Store at -20°C. Avoid freeze-thaw cycles.

References for anti-ATP2A2 antibody (ABIN152688)

Détail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Information d'application Stockage Images Haut de la page
Produit citée dans:

Leatherbury, Yu, Chatterjee, Walker, Yu, Tian, Lo: "A novel mouse model of X-linked cardiac hypertrophy." dans: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 6, pp. H2701-11, 2008

Chauvet, Tian, Husson, Grimm, Wang, Hiesberger, Hieseberger, Igarashi, Bennett, Ibraghimov-Beskrovnaya, Somlo, Caplan: "Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus." dans: The Journal of clinical investigation, Vol. 114, Issue 10, pp. 1433-43, 2004

Kjellgren, Ryan, Ohlendieck, Thornell, Pedrosa-Domellöf: "Sarco(endo)plasmic reticulum Ca2+ ATPases (SERCA1 and -2) in human extraocular muscles." dans: Investigative ophthalmology & visual science, Vol. 44, Issue 12, pp. 5057-62, 2003

Adachi, Matsui, Xu, Kirber, Lazar, Sharov, Schöneich, Cohen: "Antioxidant improves smooth muscle sarco/endoplasmic reticulum Ca(2+)-ATPase function and lowers tyrosine nitration in hypercholesterolemia and improves nitric oxide-induced relaxation." dans: Circulation research, Vol. 90, Issue 10, pp. 1114-21, 2002

Molnar, Seidler, Jona, Martonosi: "The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules." dans: Biochimica et biophysica acta, Vol. 1023, Issue 2, pp. 147-67, 1990

Background publications

Poch, Leach, Snape, Cacic, MacLennan, Lytton: "Functional characterization of alternatively spliced human SERCA3 transcripts." dans: The American journal of physiology, Vol. 275, Issue 6 Pt 1, pp. C1449-58, 1999

Hu, Yin, Zhang, Wright, Nixon, Wechsler, Spratt, Briggs: "Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching." dans: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11619-22, 1995

Sharp, McPherson, Dawson, Aoki, Campbell, Snyder: "Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 7, pp. 3051-63, 1993

Molnar, Varga, Jona, Seidler, Martonosi: "Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase." dans: Biochimica et biophysica acta, Vol. 1103, Issue 2, pp. 281-95, 1992

Ohlendieck, Briggs, Lee, Wechsler, Campbell: "Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle." dans: European journal of biochemistry / FEBS, Vol. 202, Issue 3, pp. 739-47, 1992

Plessers, Eggermont, Wuytack, Casteels: "A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 11, Issue 3, pp. 650-6, 1991

Spencer, Yu, Khan, Grover: "Expression of isoforms of internal Ca2+ pump in cardiac, smooth muscle and non-muscle tissues." dans: Biochimica et biophysica acta, Vol. 1063, Issue 1, pp. 15-20, 1991

Ohlendieck, Ervasti, Snook, Campbell: "Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma." dans: The Journal of cell biology, Vol. 112, Issue 1, pp. 135-48, 1991

Movsesian, Leveille, Krall, Colyer, Wang, Campbell: "Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles." dans: Journal of molecular and cellular cardiology, Vol. 22, Issue 12, pp. 1477-85, 1991

Briggs, Lee, Feher, Wechsler, Ohlendieck, Campbell: "Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle." dans: FEBS letters, Vol. 259, Issue 2, pp. 269-72, 1990

Jorgensen, Arnold, Pepper, Kahl, Mandel, Campbell: "A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study." dans: Cell motility and the cytoskeleton, Vol. 9, Issue 2, pp. 164-74, 1988

Images

Détail du produit anti-ATP2A2 anticorps Détail du antigène ATP2A2 Information d'application Stockage References for anti-ATP2A2 antibody (ABIN152688) Haut de la page
Images (Fournisseur)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Both norm...
Immunofluorescence (IF) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [ABIN152688] - ...
Immunofluorescence (IF) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [ABIN152688] - ...
Immunofluorescence (IF) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [ABIN152688] - ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Both norm...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Both norm...
Western Blotting (WB) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Western Blot: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Analysis of 25 ug of rat s...