anti-Xenopus laevis Nuclear Receptor Subfamily 2, Group F, Member 6 anticorps pour Immunohistochemistry (Paraffin-embedded Sections)

Recommended Nuclear Receptor Subfamily 2, Group F, Member 6 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6) Anticorps
  • EAR-2
  • EAR2
  • ERBAL2
  • AV090102
  • COUP-TF3
  • Erbal2
  • COUP(II)
  • NR2F6
  • couptf2
  • fc94g11
  • wu:fc94g11
  • zgc:77259
  • ear-2
  • ear2
  • erbal2
  • nr2f6l
  • wu:fc72d04
  • zgc:77260
  • nuclear receptor subfamily 2 group F member 6
  • nuclear receptor subfamily 2, group F, member 6
  • nuclear receptor subfamily 2, group F, member 6a
  • nuclear receptor subfamily 2 group F member 6 L homeolog
  • nuclear receptor subfamily 2, group F, member 6b
  • NR2F6
  • Nr2f6
  • nr2f6a
  • nr2f6.L
  • nr2f6
  • nr2f6b
Épitope
Ligand Binding Domain
33
13
11
8
4
2
2
2
1
1
1
1
1
1
1
Reactivité
Roussette (Chauve-souris), Boeuf (Vache), Chien, Hamster, Humain, Singe, Souris, Porc, Rat (Rattus), Xenopus laevis, Poisson zèbre (Danio rerio)
103
34
33
21
14
11
5
4
3
3
3
1
1
Hôte
Lapin
76
15
15
Clonalité
Polyclonal
Conjugué
Inconjugué
4
4
4
3
3
3
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
76
51
28
16
12
4
4
3
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Images

N° du produit ABIN1049138
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
6.813444 ABIN4306821 IHC (p) Rabbit Connectez-vous pour afficher Polyclonal 0

General

Antigène Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6) Anticorps
Épitope Ligand Binding Domain
(33), (13), (11), (8), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivité Roussette (Chauve-souris), Boeuf (Vache), Chien, Hamster, Humain, Singe, Souris, Porc, Rat (Rattus), Xenopus laevis, Poisson zèbre (Danio rerio)
(103), (34), (33), (21), (14), (11), (5), (4), (3), (3), (3), (1), (1)
Hôte Lapin
(76), (15), (15)
Clonalité
Polyclonal
Conjugué Inconjugué
(4), (4), (4), (3), (3), (3)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(76), (51), (28), (16), (12), (4), (4), (3), (1), (1), (1)
Fournisseur Connectez-vous pour afficher

Détail du produit

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Marque IHC-plus™
Specificité Human NR2F6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (100%) Opossum (94%).
Purification Immunoaffinity purified
Immunogène Synthetic 16 amino acid peptide from ligand-binding domain of human NR2F6. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (100%), Opossum (94%).

Type of Immunogen: Synthetic peptide

Détail du antigène

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Antigène
Autre désignation EAR2 / NR2F6 (NR2F6 Antibody Extrait)
Sujet Name/Gene ID: NR2F6
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: NR2F6, Erba related 2, ERBAL2, EAR-2, EAR2, ERBA-related gene-2, V-erbA-related protein 2
ID gène 2063
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Photoperiodism

Information d'application

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Indications d'application Approved: IHC, IHC-P (5 μg/mL)
Commentaires

Target Species of Antibody: Human

Procédure de l'essai The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Stockage

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Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Date de péremption 12 months

Images

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Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6) (Ligand Binding Domain) antibody (ABIN1049138) Anti-NR2F6 antibody ABIN1049138 IHC staining of human liver. Immunohistochemistry of...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6) (Ligand Binding Domain) antibody (ABIN1049138) Anti-EAR2 / NR2F6 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of fo...