anti-Humain Progesterone Receptor anticorps pour Immunohistochemistry (Formalin-fixed Sections)

Recommended Progesterone Receptor Antibody

Antigène
Progesterone Receptor (PGR) Anticorps
  • NR3C3
  • PR
  • 9930019P03Rik
  • BB114106
  • ENSMUSG00000074510
  • PR-A
  • PR-B
  • XPR-1
  • pgr
  • xpr-2
  • LOC443444
  • PGR
  • pg
  • pr
  • gb:dq017620
  • progesterone receptor
  • progesterone receptor L homeolog
  • PGR
  • Pgr
  • pgr.L
  • pgr
Épitope
AA 412-526
53
48
39
27
25
21
19
18
16
16
15
12
10
10
10
9
9
8
8
8
7
7
6
6
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain
656
115
110
25
10
9
7
6
5
3
3
3
2
2
1
1
1
1
Hôte
Lapin
381
286
3
1
Clonalité
Monoclonal
Conjugué
Cet anticorp Progesterone Receptor est non-conjugé
29
17
16
12
12
12
11
11
11
11
11
11
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
448
198
163
127
93
66
50
28
26
21
18
9
3
2
2
1
1
1
Options

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N° du produit ABIN1027517
$ 1,643.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
16.701527 ABIN1027518 IHC (f) IHC (p) Rabbit IgG AA 412-526 SP42 0
4 ABIN6167045 IHC (f) APC Mouse IgG1, kappa PR484 0
4 ABIN6167052 IHC (f) PerCP Mouse IgG1, kappa PR484 0
4 ABIN6167053 IHC (f) PE Mouse IgG1, kappa PR484 0
4 ABIN6167128 IHC (f) WB PerCP Mouse IgG1, kappa PR501 0
4 ABIN6167129 IHC (f) WB PE Mouse IgG1, kappa PR501 0
4 ABIN6167083 IHC (f) APC Mouse IgG1, kappa PR500 0
4 ABIN6167090 IHC (f) PerCP Mouse IgG1, kappa PR500 0
4 ABIN6167091 IHC (f) PE Mouse IgG1, kappa PR500 0
4 ABIN6167121 IHC (f) WB APC Mouse IgG1, kappa PR501 0
4 ABIN6167056 IHC (f) Mouse IgG1, kappa PR484 0
4 ABIN6167132 IHC (f) WB Mouse IgG1, kappa PR501 0
4 ABIN6167094 IHC (f) Mouse IgG1, kappa PR500 0
1 ABIN6167019 IHC (f) CF®405S Mouse IgG1, kappa PR484 0
1 ABIN6167021 IHC (f) CF405M Mouse IgG1, kappa PR484 0
1 ABIN6167041 IHC (f) CF®488A Mouse IgG1, kappa PR484 0
1 ABIN6167025 IHC (f) CF®543 Mouse IgG1, kappa PR484 0
1 ABIN6167029 IHC (f) CF®555 Mouse IgG1, kappa PR484 0
1 ABIN6167033 IHC (f) CF®568 Mouse IgG1, kappa PR484 0
1 ABIN6167043 IHC (f) CF®594 Mouse IgG1, kappa PR484 0

General

Antigène Progesterone Receptor (PGR) Anticorps
Épitope AA 412-526
(53), (48), (39), (27), (25), (21), (19), (18), (16), (16), (15), (12), (10), (10), (10), (9), (9), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain
(656), (115), (110), (25), (10), (9), (7), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Hôte Lapin
(381), (286), (3), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp Progesterone Receptor est non-conjugé
(29), (17), (16), (12), (12), (12), (11), (11), (11), (11), (11), (11), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(448), (198), (163), (127), (93), (66), (50), (28), (26), (21), (18), (9), (3), (2), (2), (1), (1), (1)
Fournisseur

Détail du produit anti-Progesterone Receptor anticorps

Détail du antigène Progesterone Receptor Information d'application Stockage Images
Fonction This antibody is designed for the specific localization of human PR using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.
Attributs du produit The determination of steroid hormone receptors is of great importance for therapy of patients with hormone receptor expressing tumours. Progesterone and estrogen receptor positive breast carcinoma patients have demonstrated a better response to endocrine therapy than receptor negative patients. Expression of progesterone receptors in tumour cells of human breast carcinoma points to an intact estrogen receptor and to sensitivity of this tumour to endocrine therapy. Several investigations have shown that progesterone receptor is a better prognostic marker than estrogen receptor. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Purification Ready-to-use, Prediluted, obtained from culture supernatant
Matériel non inclus All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
Immunogène Recombinant protein corresponding to amino acid residues 412-526 of human PR.
Clone SP42
Isotype IgG
Plasmids, Primers & others

Détail du antigène Progesterone Receptor

Détail du produit anti-Progesterone Receptor anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation Progesterone Receptor (PGR Antibody Extrait)
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration

Information d'application

Détail du produit anti-Progesterone Receptor anticorps Détail du antigène Progesterone Receptor Stockage Images Haut de la page
Commentaires

Staining pattern: Nuclear. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from breast carcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for PR.

Protocole Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Procédure de l'essai Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:50 – 1:200
Incubation: 30 min, RT
Control Tissue: Breast carcinoma
Restrictions For Research Use only

Stockage

Détail du produit anti-Progesterone Receptor anticorps Détail du antigène Progesterone Receptor Information d'application Images Haut de la page
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.

Images

Détail du produit anti-Progesterone Receptor anticorps Détail du antigène Progesterone Receptor Information d'application Stockage Haut de la page
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Progesterone Receptor (PGR) (AA 412-526) antibody (ABIN1027517) 10 × 20 × IHC of Progesterone Receptor (clone SP42) on FFPE breast carcinoma tissue. ...