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Souris Prolactin EZ Set™ ELISA Kit (DIY Antibody Pairs)

ELISA Reactivité: Souris ELISA unconjugated AA 32-228 Hôte: Lapin
N° du produit ABIN5510772
  • Antigène Tous les produits Prolactin (PRL)
    Prolactin (PRL)
    Épitope
    AA 32-228
    Reactivité
    Souris
    Méthode de détection
    Colorimetric
    Hôte
    • 2
    • 1
    Lapin
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Prolactin in Mouse samples.
    Marque
    EZ-Set™
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: L32-C228
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Ingrédients
    Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
    Matériel non inclus
    1. Microplate reader in standard size.
    2. Automated plate washer.
    3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
    4. Clean tubes and Eppendorf tubes.
    5. Washing buffer (neutral PBS or TBS).
  • Commentaires

    The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
    The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
    The EZ Set™ Accessory Kit provides:
    Antibody diluent buffer
    ABC diluent buffer
    Sample diluent buffer
    TMB
    TMB stop solution
    5x 96 well plates, plate sealers

    Restrictions
    For Research Use only
  • Buffer
    Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
    Date de péremption
    12 months
  • Antigène
    Prolactin (PRL)
    Autre désignation
    Prl (PRL Produits)
    Synonymes
    PRL Matched Antibody Pair, prolactin Matched Antibody Pair, PRLB Matched Antibody Pair, PRLSD1 Matched Antibody Pair, Prl1a1 Matched Antibody Pair, Prol Matched Antibody Pair, RATPRLSD1 Matched Antibody Pair, RNPROL Matched Antibody Pair, AV290867 Matched Antibody Pair, prl Matched Antibody Pair, prolactin Matched Antibody Pair, prolactin, gene 2 L homeolog Matched Antibody Pair, PROLACTIN protein Matched Antibody Pair, prolactin-like Matched Antibody Pair, PRL Matched Antibody Pair, prl.2.L Matched Antibody Pair, Prl Matched Antibody Pair, LOC100136792 Matched Antibody Pair, LOC100136580 Matched Antibody Pair, PROLACTIN Matched Antibody Pair, LOC101843376 Matched Antibody Pair
    Sujet

    Protein Function: Prolactin acts primarily on the mammary gland by promoting lactation.

    Background: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

    Synonyms: Prolactin,PRL,Prl,

    Full Gene Name: Prolactin

    Cellular Localisation: Secreted.
    UniProt
    P06879
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