When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
SLC12A4
(Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))
Synonymes
KCC1 Peptide, kcc1 Peptide, SLC12A4 Peptide, AW546649 Peptide, RBCKCC1 Peptide, Kcc1 Peptide, solute carrier family 12 member 4 Peptide, solute carrier family 12 (potassium/chloride transporter), member 4 L homeolog Peptide, solute carrier family 12, member 4 Peptide, SLC12A4 Peptide, slc12a4.L Peptide, Slc12a4 Peptide