TIMP2 Protein (AA 30-220, partial) (GST tag)

N° du produit ABIN1095681

Détail du produit

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Type de proteíne: Recombinant

Attributs du protein: AA 30-220, partial

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette TIMP2 protéine est marqué à la GST tag.

Application: ELISA

Séquence: SPVHPQQAFC NADVVIRAKA VSEKEVDSGN DIYGNPIKRI QYEIKQIKMF KGPEKDIEFI YTAPSSAVCG VSLDVGGKKE YLIAGKAEGD GKMHITLCDF IVPWDTLSTT QKKSLNHRYQ MGCECKITRC PMIPCYISSP DECLWMDWVT EKNINGHQAK FFACIKRSDG SCAWYRGAAP PKQEFLDIED P

Attributs du produit: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Pureté: 90 %

Information d'application

Commentaires:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Conseil sur la manipulation: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Stock: -20 °C

Stockage commentaire: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Référence

Stetler-Stevenson, Brown, Onisto, Levy, Liotta: "Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues." dans: The Journal of biological chemistry, Vol. 265, Issue 23, pp. 13933-8, (1990) (PubMed).

Boone, Johnson, De Clerck, Langley: "cDNA cloning and expression of a metalloproteinase inhibitor related to tissue inhibitor of metalloproteinases." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2800-4, (1990) (PubMed).

Détail du antigène

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Autre désignation: Metalloproteinase inhibitor 2 protein (TIMP2 Produits)

Sujet: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Poids moléculaire: 48.8 kD

UniProt: P16035

Pathways: cAMP Metabolic Process