MYL6 Protein (AA 3-151, partial) (GST tag)

N° du produit ABIN1047437

Détail du produit

Antigène: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Type de proteíne: Recombinant

Attributs du protein: AA 3-151, partial

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette MYL6 protéine est marqué à la GST tag.

Application: ELISA

Séquence: DFTEDQTAEF KEAFQLFDRT GDGKILYSQC GDVMRALGQN PTNAEVLKVL GNPKSDEMNV KVLDFEHFLP MLQTVAKNKD QGTYEDYVEG LRVFDKEGNG TVMGAEIRHV LVTLGEKMTE EEVEMLVAGH EDSNGCINYE AFVRHILSG

Attributs du produit: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Pureté: 90 %

Information d'application

Commentaires:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Conseil sur la manipulation: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Stock: -20 °C

Stockage commentaire: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Référence

Watanabe, Kohri, Takaishi, Horie, Higashihara: "Molecular cloning and sequencing of myosin light chains in human megakaryoblastic leukemia cells." dans: Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi, Vol. 37, Issue 1, pp. 25-38, (2001) (PubMed).

Bora, Bora, Wu, Kaplan, Lange: "Molecular cloning, sequencing, and characterization of smooth muscle myosin alkali light chain from human eye cDNA: homology with myocardial fatty acid ethyl ester synthase-III cDNA." dans: Genomics, Vol. 19, Issue 1, pp. 186-8, (1994) (PubMed).

Hailstones, Gunning: "Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function." dans: Molecular and cellular biology, Vol. 10, Issue 3, pp. 1095-104, (1990) (PubMed).

Lenz, Lohse, Seidel, Arnold: "The alkali light chains of human smooth and nonmuscle myosins are encoded by a single gene. Tissue-specific expression by alternative splicing pathways." dans: The Journal of biological chemistry, Vol. 264, Issue 15, pp. 9009-15, (1989) (PubMed).

Détail du antigène

Antigène: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Autre désignation: Myosin light polypeptide 6 protein (MYL6 Produits)

Synonymes: ESMLC Protein, LC17 Protein, LC17-GI Protein, LC17-NM Protein, LC17A Protein, LC17B Protein, MLC-3 Protein, MLC1SM Protein, MLC3NM Protein, MLC3SM Protein, zgc:103624 Protein, MLC3nm Protein, Myln Protein, myosin light chain 6 Protein, myosin, light chain 6, alkali, smooth muscle and non-muscle Protein, myosin light chain 6 L homeolog Protein, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle Protein, MYL6 Protein, Myl6 Protein, myl6 Protein, myl6.L Protein

Sujet: Regulatory light chain of myosin. Does not bind calcium.

Poids moléculaire: 44.1 kD

UniProt: P60660