CD73 Protein (AA 27-547) (His tag)

N° du produit ABIN1095954

Détail du produit

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Type de proteíne: Recombinant

Attributs du protein: AA 27-547

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette CD73 protéine est marqué à la His tag.

Fonction: Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (C-6His)

Séquence: WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHA DETFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI KVDHHHHHH

Attributs du produit: Recombinant Human 5'-Nucleotidase/NT5E is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Trp27-Lys547) of Human NT5E fused with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 120 mM NaCl,4 mM CaCl2, 20 % Glycerol, pH 7.5.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: cd73 (NT5E Produits)

Synonymes: CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein

Sub Type: Fusionprotein

Sujet: CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Alternative Names: 5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, CD73, NT5E, NT5, NTE

Poids moléculaire: 58.8 kDa

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process