ATG10 Protein (AA 1-190) (His tag,T7 tag)

N° du produit ABIN1096062

Cette protéine Recombinant ATG10 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour ATG10 Protein (AA 1-190) (His tag,T7 tag) (ABIN1096062)

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-190

Purification/Conjugué: Cette ATG10 protéine est marqué à la His tag,T7 tag.

Fonction: Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)

Séquence: MASMTGGQQM GRGSMEEDEF IGEKTFQRYC AEFIKHSQQI GDSWEWRPSK DCSDGYMCKI HFQIKNGSVM SHLGASTHGQ TCLPMEEAFE LPLDDCEVIE TAAASEVIKY EYHVLYSCSY QVPVLYFRAS FLDGRPLTLK DIWEGVHECY KMRLLQGPWD TITQQEHPIL GQPFFVLHPC KTNEFMTPVL KNSQKINKNV NYITLEHHHH HH

Attributs du produit:

Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 8.0.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Autre désignation: Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10

Sujet: Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr190) of Human ATG10 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28 kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.

Poids moléculaire: 24.5 kDa

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy