POLB Protein (AA 2-335) (His tag)

N° du produit ABIN1096540

Détail du produit

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Type de proteíne: Recombinant

Attributs du protein: AA 2-335

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette POLB protéine est marqué à la His tag.

Fonction: Recombinant Human DNA Polymerase β (C-6His)

Séquence: MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSEVEHHH HHH

Attributs du produit: Recombinant Human DNA Polymerase beta is produced with our E. coli expression system. The target protein is expressed with sequence (Ser2-Glu335) of Human DNA Polymerase beta.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: DNA Polymerase beta (POLB Produits)

Synonymes: wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein

Sujet: Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta.
Alternative Names: DNA Polymerase Beta, POLB

Poids moléculaire: 39.23 kDa

UniProt: P06746

Pathways: Réparation de l'ADN