ASF1A Protein (AA 1-204) (His tag,T7 tag)

N° du produit ABIN1096822

Détail du produit

Antigène: ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-204

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ASF1A protéine est marqué à la His tag,T7 tag.

Fonction: Recombinant Human Histone Chaperone ASF1A/ASF1A (C-6His, N-T7 tag)

Séquence: MASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH

Attributs du produit: Recombinant Human Histone Chaperone ASF1A/ASF1A is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Met204) of Human ASF1A fused with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 150 mM NaCl, pH 8.0.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 3 months

Détail du antigène

Antigène: ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))

Autre désignation: Histone Chaperone ASF1A/ASF1A (ASF1A Produits)

Synonymes: CGI-98 Protein, CIA Protein, HSPC146 Protein, 2310079C17Rik Protein, C79052 Protein, ASF1 Protein, ASF1A Protein, XAsf1 Protein, asf1 Protein, asf1-a Protein, asf1a Protein, asf1a-a Protein, asf1aa Protein, asf1ab Protein, anti-silencing function 1A histone chaperone Protein, anti-silencing function 1A histone chaperone L homeolog Protein, ASF1A Protein, Asf1a Protein, asf1a.L Protein

Sub Type: Fusionprotein

Sujet: Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Alternative Names: Histone Chaperone ASF1A, Anti-Silencing Function Protein 1 Homolog A, hAsf1, hAsf1a, CCG1-Interacting Factor A, CIA, hCIA, ASF1A

Poids moléculaire: 25.4 kDa

UniProt: Q9Y294