MMP1 Protein (AA 20-469) (His tag)

N° du produit ABIN1097152

Détail du produit

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Type de proteíne: Recombinant

Attributs du protein: AA 20-469

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette MMP1 protéine est marqué à la His tag.

Fonction: Recombinant Human Matrix Metalloproteinase-1/MMP-1 (C-6His)

Séquence: FPATLETQEQ DVDLVQKYLE KYYNLKNDGR QVEKRRNSGP VVEKLKQMQE FFGLKVTGKP DAETLKVMKQ PRCGVPDVAQ FVLTEGNPRW EQTHLTYRIE NYTPDLPRAD VDHAIEKAFQ LWSNVTPLTF TKVSEGQADI MISFVRGDHR DNSPFDGPGG NLAHAFQPGP GIGGDAHFDE DERWTNNFRE YNLHRVAAHE LGHSLGLSHS TDIGALMYPS YTFSGDVQLA QDDIDGIQAI YGRSQNPVQP IGPQTPKACD SKLTFDAITT IRGEVMFFKD RFYMRTNPFY PEVELNFISV FWPQLPNGLE AAYEFADRDE VRFFKGNKYW AVQGQNVLHG YPKDIYSSFG FPRTVKHIDA ALSEENTGKT YFFVANKYWR YDEYKRSMDP GYPKMIAHDF PGIGHKVDAV FMKDGFFYFF HGTRQYKFDP KTKRILTLQK ANSWFNCRKN VDHHHHHH

Attributs du produit: Recombinant Human Matrix Metalloproteinase-1/MMP-1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Phe20-Asn469) of Human MMP-1 fused with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 0.05 % Brij35, pH 5.5.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Autre désignation: mmp-1 (MMP1 Produits)

Synonymes: CLG Protein, CLGN Protein, Mmp1a Protein, CG4859 Protein, Dm1-MMP Protein, Dmel\\CG4859 Protein, MMP-1 Protein, MMP1 Protein, Mmp 1 Protein, dMMP1 Protein, dm1-MMP Protein, dmmp1 Protein, l(2)k04809 Protein, mmp1 Protein, Mmp1 Protein, Mcol-A Protein, Mcola Protein, col4 Protein, mmp18 Protein, Clgn Protein, matrix metallopeptidase 1 Protein, Matrix metalloproteinase 1 Protein, matrix metalloproteinase 1 Protein, matrix metallopeptidase 1 (interstitial collagenase) Protein, matrix metallopeptidase 1a (interstitial collagenase) Protein, matrix metallopeptidase 1 S homeolog Protein, interstitial collagenase Protein, matrix metallopeptidase 8 L homeolog Protein, MMP1 Protein, Mmp1 Protein, RB11133 Protein, Mmp1a Protein, mmp1.S Protein, LOC100727966 Protein, mmp8.L Protein

Sub Type: Fusionprotein

Sujet: Matrix Metalloproteinase-1 (MMP-1) is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 contains several distinct domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin like) domain. MMP-1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, alpha1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL1, IGFBP3, IGFBP5, pro-MMP2, and pro-MMP9. A significant role of MMP1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into 3/4, 1/4 fragments. MMP1 may also be involved in enzyme cascades, cytokine regulation and cell surface molecule modulation.
Alternative Names: Interstitial Collagenase, Fibroblast Collagenase, Matrix Metalloproteinase-1, MMP-1, MMP1, CLG

Poids moléculaire: 52.88 kDa

UniProt: P03956