Recombinant Human alpha-Soluble NSF Attachment Protein/SNAP-alpha produced by E.coli expression system.Target protein is expressed with sequence (Met1-Arg295) of Human SNAPalpha (Uniprot #P54920) fused with a 6His tag at the N-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
NAPA
Origine: Rat
Hôte: Levure
Recombinant
> 90 %
ELISA
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
4 °C/-20 °C/-80 °C
Stockage commentaire
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Sub Type
Fusionprotein
Sujet
Alpha-Soluble NSF Attachment Protein (SNAP-alpha) is a member of the SNAP (Soluble NSF Attachment Protein) family. SNAP-alpha interacts with PRKCABP and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. SNAP-alpha is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. SNAP-alpha is in charge of the binding of NSF and therefore the formation of a 20S fusion particle. Alternative Names: Alpha-Soluble NSF Attachment Protein, SNAP-Alpha, N-Ethylmaleimide-Sensitive Factor Attachment Protein Alpha, NAPA, SNAPA