TRAIL Protein (AA 115-281)

N° du produit ABIN1097691

Détail du produit

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 115-281

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Fonction: Recombinant Human TRAIL/TNFSF10/CD253 (Arg115-Gly281)

Séquence: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

Attributs du produit: Recombinant Human Tumor Necrosis Factor Ligand Superfamily Member 10/TNFSF10 produced by our E. coli expression system is composed with the C- terminal extracellular domain including THD. The target protein is expressed with sequence (R115-G281) of Human TNFSF10.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Commentaires:

Biological activity: ED50 is less than 2 ng/ml as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.Specific Activity of 5.0 x 106 IU/mg.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 150 mM NaCl.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 3 months

Détail du antigène

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: trail-protein (TNFSF10 Produits)

Synonymes: APO2L Protein, Apo-2L Protein, CD253 Protein, TL2 Protein, TRAIL Protein, A330042I21Rik Protein, AI448571 Protein, APO-2L Protein, Ly81 Protein, Trail Protein, tl2 Protein, apo2l Protein, cd253 Protein, trail Protein, apo-2l Protein, trail1 Protein, xtrail1 Protein, tnfsf10l2 Protein, zgc:92320 Protein, TNLG6A Protein, TNF superfamily member 10 Protein, tumor necrosis factor (ligand) superfamily, member 10 Protein, tumor necrosis factor superfamily member 10 Protein, TNF superfamily member 10 S homeolog Protein, TNFSF10 Protein, Tnfsf10 Protein, tnfsf10.S Protein, tnfsf10 Protein

Sujet: Human TNFSF10 is a type II transmembrane protein with an intracellular N-terminus and a ‘TNF homology domain’ (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct receptors. Two of these receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2), are plasma membrane proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells.
Alternative Names: Tumor Necrosis Factor Ligand Superfamily Member 10, Apo-2 Ligand, Apo-2L, TNF-Related Apoptosis-Inducing Ligand, Protein TRAIL, CD253, TNFSF10, APO2L, TRAIL

Poids moléculaire: 19.5 kDa

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity