ATG10 Protein (AA 1-220) (His tag)

N° du produit ABIN1098406

Protéine Recombinant ATG10 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour ATG10 Protein (AA 1-220) (His tag) (ABIN1098406)

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-220

Purification/Conjugué: Cette ATG10 protéine est marqué à la His tag.

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP

Purification: > 95% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Synonyms: Ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol,1 mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Autre désignation: ATG10

Sujet: Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 27.7 kDa (243aa) confirmed by MALDI-TOF

NCBI Accession: NP_113670

Pathways: ER-Nucleus Signaling, Autophagy