CCL2 Protéine

N° du produit ABIN1305099

Détail du produit

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Type de proteíne: Recombinant

Origine: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Marque: BD Pharmingen™

Specificité: Upon initial thawing, recombinant rat MCP-1 (MN 555110) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible affects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Attributs du produit: Recombinant rat MCP-1 (MN 555110) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing glycerol and 1.0 mg/ml bovine serum albumin, with no preservatives. Recombinant rat MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant rat MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing glycerol and 1.0 mg/mL bovine serum albumin, with no preservatives. Recombinant rat MCP-1 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Pureté: ≥ 95 %

Stérilité: 0.22 μm filtered

niveau d'endotoxine: The endotoxin level is ≤ 0.1 ng per µg of rat MCP-1, as measured in a chromogenic LAL assay.

Information d'application

Indications d'application: ELISA Standard: Recombinant rat MCP-1 (MN 555110) can be useful as a quantitative standard for measuring rat MCP-1 protein levels using sandwich ELISA with the purified C4 antibody (Cat. No. 555072) as a capture antibody and the biotinylated B4 antibody (Cat. No. 555074) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant rat MCP-1 from 2000-5 pg/mL to be included for each ELISA plate. For measuring rat MCP-1 in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Rat MCP-1 Set (Cat. No. 555130).
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range encompassing an ED50 = 60 - 600 ng/mL has previously been reported using THP-1 as indicator cells utilizing a calcium flux assay. Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant rat MCP-1 can be used as a blocking control to demonstrate the specificity of MCP-1 staining by the PE-conjugated format (Cat. No.554443) of the 2H5 Anti-Rat MCP-1 antibody. Investigators are advised that this blocking application is not routinely tested for this material. The use of staining controls for the immunofluorescent staining and flow cytometric analysis of cytokine producing cells has been previously described (Prussin et al.).

Commentaires:

BD Pharmingen™ Recombinant Rat MCP-1 - Reactivity Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Agent conservateur: Azide free

Conseil sur la manipulation: Rat Monocyte Chemoattractant Protein (MCP-1) is a member of the C-C chemokine superfamily and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells.3 MCP-1 has chemoattractant activity for monocytes, lymphocytes and basophils but is not active for neutrophils. Recombinant rat MCP-1 is a highly glycosylated protein ranging in size from 27 - 30 kD as measured by SDS-PAGE analysis. Furthermore, there is evidence from experimental animal models that MCP-1 can suppress tumor formation by attracting monocytes to the tumor site.

Stock: -80 °C

Stockage commentaire: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Référence

Haelens, Wuyts, Proost, Struyf, Opdenakker, van Damme: "Leukocyte migration and activation by murine chemokines." dans: Immunobiology, Vol. 195, Issue 4-5, pp. 499-521, (1997) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Rollins, Sunday: "Suppression of tumor formation in vivo by expression of the JE gene in malignant cells." dans: Molecular and cellular biology, Vol. 11, Issue 6, pp. 3125-31, (1991) (PubMed).

Yoshimura, Takeya, Takahashi: "Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines." dans: Biochemical and biophysical research communications, Vol. 174, Issue 2, pp. 504-9, (1991) (PubMed).

Capsoni, Minonzio, Ongari, Zanussi: "A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes." dans: Journal of immunological methods, Vol. 120, Issue 1, pp. 125-31, (1989) (PubMed).

Détail du antigène

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP-1 (CCL2 Produits)

Synonymes: GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein

Sujet: Rat Monocyte Chemoattractant Protein (MCP-1) is a member of the C-C chemokine superfamily and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells.3 MCP-1 has chemoattractant activity for monocytes, lymphocytes and basophils but is not active for neutrophils. Recombinant rat MCP-1 is a highly glycosylated protein ranging in size from 27 - 30 kD as measured by SDS-PAGE analysis. Furthermore, there is evidence from experimental animal models that MCP-1 can suppress tumor formation by attracting monocytes to the tumor site.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2