CCL2 Protéine

N° du produit ABIN1305101

Détail du produit

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Marque: BD Pharmingen™

Specificité: Bioactivity: This material has been reported to have bioactivity when measured by Ca 2+ Flux Assay, using Human THP-1cells. The EC50 is commonly in the range of 20-350 ng/ml. Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Attributs du produit: Recombinant human MCP-1 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The human MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant human MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant human MCP-1 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Pureté: ≥ 95 %

Stérilité: 0.22 μm filtered

niveau d'endotoxine: The endotoxin level is ≤ 0.1 ng per µg of human MCP-1, as measured in a chromogenic LAL assay.

Information d'application

Indications d'application: ELISA Standard: The human MCP-1 (Cat. No. 554620) is useful as a quantitative standard for measuring human MCP-1 protein levels in a sandwich ELISA with the purified 10F7 antibody (Cat. No. 555055) as a capture antibody and the biotinylated 5D3-F7 (Cat. No. 554664) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human MCP-1 standard from 2000-4 pg/ml should be included in each ELISA plate.

Commentaires:

BD Pharmingen™ Recombinant Human MCP-1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Agent conservateur: Azide free

Conseil sur la manipulation: Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.

Stock: -80 °C

Stockage commentaire: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Référence

Rollins: "Chemokines." dans: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).

Rollins, Stier, Ernst, Wong: "The human homolog of the JE gene encodes a monocyte secretory protein." dans: Molecular and cellular biology, Vol. 9, Issue 11, pp. 4687-95, (1990) (PubMed).

Détail du antigène

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP-1 (CCL2 Produits)

Synonymes: GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein

Sujet: Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2