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TNF alpha Protéine

TNF alpha Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant ≥ 95 % ELISA, Func, ICFC, BR Active
N° du produit ABIN1305135
  • Antigène Voir toutes TNF alpha Protéines
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Origine
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    Souris
    Source
    • 61
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    Escherichia coli (E. coli)
    Application
    ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)
    Marque
    BD Pharmingen™
    Specificité
    Biological Activity: Activity Test: MTT/Actinomycin D Cytolysis assay using L929 indicator cells at 2 x 10^5 cells/ml
    Specific Activity: 0.2 - 5.0 x 10^9 Units /mg
    ED50: 2 - 50 pg/ml
    Observed dose response relationship: 80 fold A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    Attributs du produit
    Recombinant mouse TNF is supplied as a frozen aqueous buffered solution containing biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse TNF was purified and found to be > 95% pure by SDS-PAGE, and an absorption assay based on the Beers-Lambert Law.
    Purification
    Recombinant mouse TNF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives. Recombinant mouse TNF is ≥ 95 % pure as determined by SDS-PAGE, and an absorption assay based on the Beers-Lambert Law.
    Pureté
    ≥ 95 %
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    The endotoxin level is ≤ 0.1 ng per µg of TNF, as measured in a chromogenic LAL assay.
    Top Product
    Discover our top product TNF alpha Protéine
  • Indications d'application
    ELISA Standard: Recombinant mouse TNF is useful as a quantitative standard for measuring mouse TNF protein levels in an TNF specific sandwich ELISA with the purified G281-2626 antibody (Cat. No. 551225) as a capture antibody and the biotinylated MP6-XT3 (Cat. No. 554415) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse TNF standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
    Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse TNF in serum or plasma the BD OptEIA™ Mouse TNF ELISA Sets (Cat. No. 555268 or 558534) and BD OptEIA™ Mouse TNF ELISA Kit (Cat. No. 559732 or 560478) are specially formulated and recommended.
    Ligand Blocking Control for Immunfuorescent Staining of Cytokines: Recombinant mouse TNF can be used as a blocking control to demonstrate specificity of TNF staining by the directly conjugated-MP6-XT22 antibody. To perform this control, the fluorochrome-conjugated antibody can be preincubated with 0.05 - 0.5 µg of recombinant mouse TNF for 20 minutes at 4°C, prior to staining. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
    Commentaires

    BD Pharmingen™ Recombinant Mouse TNF - Reactivity Ms

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    200 μg/mL
    Buffer
    Frozen aqueous buffered solution containing BSA.
    Agent conservateur
    Azide free
    Conseil sur la manipulation
    This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier protein concentrations of ≥ 1 mg/ml. For use as an ELISA standard, we recommend carrier protein concentrations of 5 - 10 mg/ml. The product should not be diluted to less than 50 µg/ml for long term storage. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
    Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect exper imental results due to toxicity, high endotoxin levels or possible blocking activity.
    Stock
    -80 °C
    Stockage commentaire
    Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

    Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

    Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).

  • Antigène
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Autre désignation
    TNF (TNF alpha Produits)
    Synonymes
    DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
    Sujet
    Tumor Necrosis Factor (TNF, aka TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Mouse TNF is a differentially glycosylated protein containing 156 amino acid residues.
    Pathways
    Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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