TNF alpha Protein (AA 1-233) (GST tag)

N° du produit ABIN1323120

Détail du produit

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Attributs du protein: AA 1-233

Origine: Humain

Source: Wheat germ

Purification/Conjugué: Cette TNF alpha protéine est marqué à la GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Fonction: TNF (Human) Recombinant Protein (P01)

Séquence: MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL

Attributs du produit: Human TNF full-length ORF ( AAH28148, 1 a.a. - 233 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Stockage

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -80 °C

Stockage commentaire: Best use within three months from the date of receipt of this protein.

Référence

Kwak, Han, Bae: "Aspalathin and nothofagin from rooibos (Aspalathus linearis) inhibit endothelial protein C receptor shedding in vitro and in vivo." dans: Fitoterapia, Vol. 100, pp. 179-86, (2015) (PubMed).

Ku, Kim, Bae: "Piperlonguminine downregulates endothelial protein C receptor shedding in vitro and in vivo." dans: Inflammation, Vol. 37, Issue 2, pp. 435-42, (2014) (PubMed).

Ku, Kim, Han, Bae: "Antithrombotic activities of epi-sesamin in vitro and in vivo." dans: The American journal of Chinese medicine, Vol. 41, Issue 6, pp. 1313-27, (2013) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF (TNF alpha Produits)

Sujet: Full Gene Name: tumor necrosis factor (TNF superfamily, member 2)
Synonyms: DIF,TNF-alpha,TNFA,TNFSF2

ID gène: 7124

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome