Netrin 1 Protein (NTN1) (AA 25-604) (DYKDDDDK Tag)

N° du produit ABIN1344382

Détail du produit

Antigène: Netrin 1 (NTN1)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 25-604

Origine: Souris, Humain, Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette Netrin 1 protéine est marqué à la DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Specificité: Binds to human, mouse and rat UNC5B.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Attributs du produit: Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.01EU/μg purified protein (LAL test, Lonza).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile distilled water.

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Date de péremption: 6 months

Référence

Ozmadenci, Féraud, Markossian, Kress, Ducarouge, Gibert, Ge, Durand, Gadot, Plateroti, Bennaceur-Griscelli, Scoazec, Gil, Deng, Bernet, Mehlen, Lavial: "Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance." dans: Nature communications, Vol. 6, pp. 7398, (2015) (PubMed).

Détail du antigène

Antigène: Netrin 1 (NTN1)

Autre désignation: Netrin-1 (NTN1 Produits)

Synonymes: ntn1-A Protein, Netrin-1 Protein, NTN1 Protein, unc-6 Protein, MGC147073 Protein, NTN1L Protein, AI561871 Protein, BC019633 Protein, netrin-1 Protein, netrin 1 L homeolog Protein, netrin 1 Protein, ntn1.L Protein, NTN1 Protein, ntn1 Protein, Ntn1 Protein

Sujet: Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.

Poids moléculaire: ~80kDa (SDS-PAGE)

UniProt: O95631

Pathways: Regulation of Cell Size