Flagellin Protein (FliC)

N° du produit ABIN1344458

Détail du produit

Antigène: Flagellin (FliC)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Souris

Source: Salmonella typhimurium

Application: SDS-PAGE (SDS)

Specificité: Binds to human and mouse TLR5 (Toll-like receptor 5).

Réactivité croisée: Humain, Souris

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.01EU/μg purified protein (LAL test, Lonza).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Flagellin (FliC)

Autre désignation: Flagellin (FliC Produits)

Classe de substances: Tag

Sujet: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

UniProt: P06179

Pathways: Inflammasome