Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

PDGF-BB Homodimer (Homodimer) (Active) Protéine

Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE. Visualized by silver stain Active
N° du produit ABIN1589629
  • Antigène
    PDGF-BB Homodimer
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    Homodimer
    Origine
    • 9
    • 5
    • 3
    Humain
    Source
    • 12
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Séquence
    SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
    Attributs du produit
    Length (AA): 109
    Chromosomal location: 22q13.1
    The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
    Pureté
    > 95 % by SDS-PAGE. Visualized by silver stain
    niveau d'endotoxine
    < 0.1 ng per µg of PDGF-BB
  • Commentaires

    Cytokines & Growth Factors

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    The lyophilized PDGF-BB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1%) is recommended.
    Buffer
    50 mM acetic acid
    Conseil sur la manipulation
    Centrifuge vial prior to opening.
    Stock
    -20 °C
    Stockage commentaire
    The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted PDGF-BB is best stored at -20 °C to -70 °C.
  • Rezzola, Dal Monte, Belleri, Bugatti, Chiodelli, Corsini, Cammalleri, Cancarini, Morbidelli, Oreste, Bagnoli, Semeraro, Presta: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." dans: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).

  • Antigène
    PDGF-BB Homodimer
    Autre désignation
    PDGF-BB
    Synonymes
    PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
    Sujet
    PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
    Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, INN=Becaplermin
    Poids moléculaire
    24.3 kDa
    NCBI Accession
    NP_002599, NM_002608
    UniProt
    P01127
Vous êtes ici:
Support technique