MANF Protein (AA 25-182) (His tag)

N° du produit ABIN1691494

Détail du produit

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Type de proteíne: Recombinant

Attributs du protein: AA 25-182

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette MANF protéine est marqué à la His tag.

Fonction: Recombinant Human MANF/ARMET (C-6His)

Séquence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDLHH HHHH

Attributs du produit: Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor/MANF is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu25­Leu182) of Human MANF fused with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 5 months

Détail du antigène

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: MANF (MANF Produits)

Synonymes: ARMET Protein, ARP Protein, 3230402M22Rik Protein, AA407711 Protein, AA408789 Protein, AA673178 Protein, Armet Protein, D17914 Protein, D18Mgi17 Protein, MANF Protein, RBM15B Protein, ARP-like Protein, CG7013 Protein, DmMANF Protein, Dmel\\CG7013 Protein, anon-EST:ParkEST188 Protein, arp Protein, armet Protein, im:6909481 Protein, zgc:153327 Protein, manf Protein, mesencephalic astrocyte derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor Protein, RNA binding motif protein 15B Protein, Mesencephalic astrocyte-derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor S homeolog Protein, MANF Protein, Manf Protein, RBM15B Protein, manf Protein, manf.S Protein

Sub Type: Fusionprotein

Sujet: Mesencephalic astrocyte-derived neurotrophic factor(MANF) is a secreted protein which belongs to the ARMET family. MANF selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. MANF enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. MANF inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF and a homologous protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. MANF expression was widespread in the nervous system and non-neuronal tissues.
Alternative Names: Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET, ARMET, ARP

Poids moléculaire: 21.5 kDa

UniProt: P55145