Recombinant Human D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg343) of Human BDH1 fused with a 6His tag at the N-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
BDH1
Origine: Rat
Hôte: Levure
Recombinant
> 90 %
ELISA
Restrictions
For Research Use only
Format
Liquid
Reconstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 50 mM Tris, 1 mM DTT, pH 7.5.
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
-80 °C
Stockage commentaire
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Date de péremption
6 months
Antigène
BDH1
(3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))
zgc:163053 Protein, BDH Protein, SDR9C1 Protein, 2310032J20Rik Protein, AI327223 Protein, Bdh Protein, 3-hydroxybutyrate dehydrogenase 1 Protein, 3-hydroxybutyrate dehydrogenase 1 S homeolog Protein, 3-hydroxybutyrate dehydrogenase, type 1 Protein, 3-hydroxybutyrate dehydrogenase, type 1 A Protein, BDH1 Protein, bdh1.S Protein, bdh1 Protein, Bdh1 Protein, BDH1A Protein
Sub Type
Fusionprotein
Sujet
D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism. Alternative Names: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial, BDH, 3-Hydroxybutyrate Dehydrogenase, BDH