desert Hedgehog Protein (AA 23-198, Cys23IleIle-Mutant)

N° du produit ABIN2017734

Détail du produit

Antigène: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 23-198, Cys23IleIle-Mutant

Origine: Humain

Source: Escherichia coli (E. coli)

Attributs du produit: ED50 < 10 μg/, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of > 100 units/mg.
AA 23-198 (Cys23IleIle), with and without an N-terminal Met

Pureté: > 95 % by SDS-PAGE and HPLC analyses.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant Human Hedgehog homolog(rhDHH) remains stable up to 6 months at-80 °C from date of receipt. Upon reconstitution, rhDHH remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Abstract: DHH Produits

Synonymes: GDXYM Protein, HHG-3 Protein, SRXY7 Protein, C78960 Protein, Xchh Protein, chh Protein, chh-a Protein, dhh Protein, dhh-1 Protein, dhh-A Protein, hh4 Protein, x-chh Protein, desert hedgehog Protein, desert hedgehog L homeolog Protein, desert hedgehog S homeolog Protein, DHH Protein, dhh Protein, Dhh Protein, dhh.L Protein, dhh.S Protein

Classe de substances: Species

Sujet: Desert hedgehog protein (DHH) is a member of the Hedgehog family which encodes signaling molecules that play an important role in regulating morphogenesis. It is predicted to be made as a precursor that is auto-catalytically cleaved, the N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH may be involved in both male gonadal differentiation and perineurial development. DHH binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). It induces steroidogenic factor 1(SF1), which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. Recombinant human Desert hedgehog (DHH) produced in Escherichia Coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule, rhDHH has a molecular mass of around 20 kDa analyzed by reducing SDS-PAGE.
Synonyms: DHH, Human

Poids moléculaire: 20 kDa, observed by reducing SDS-PAGE.

Pathways: Signalisation Hedgehog